热性惊厥儿童白细胞介素-1受体拮抗剂基因多态性分析  

Analysis of Interleukin-1 Recepetor Antagonist Gene Polymorphisms of Children with Febrile Seizwres

作　　者：李淑华[1] 胡巢凤[2] 马文成[1] 

机构地区：[1]广州市儿童医院心血管内科,广州510120 [2]暨南大学医学院病理生理学教研室,广州510632

出　　处：《实用儿科临床杂志》2007年第7期521-522,共2页Journal of Applied Clinical Pediatrics

基　　金：国家中医药管理局三级重点实验室--病理生理实验室开放基金项目资助

摘　　要：目的分析白细胞介素-1受体拮抗剂基因(IL-1RN)与儿童热性惊厥(FS)遗传易感性的关系。方法采用病例-对照研究方法收集52例FS病例组和53例对照组外周血样本和流行病学调查资料,提取基因组DNA,采用聚合酶链反应(PCR)方法直接检测IL-1RN基因多态性。结果采自广东省汉族儿童的105例样本中仅发现3种IL-1RN基因型,分别是Ⅰ/Ⅰ、Ⅰ/Ⅱ、Ⅱ/Ⅳ,各基因型在病例与对照组分布无显著性差异。等位基因Ⅰ、Ⅱ和Ⅳ在病例组分布频率分别是95.2%、3.8%、1.0%,在对照组中的分布频率分别是84.9%、10.4%、4.7%,IL-1RN等位基因Ⅰ的频率明显高于对照组(P<0.05)。结论IL-1RN等位基因Ⅰ可能与儿童FS易感性有关。Objective To evaluate the relationship between children interleukin-1 receptor antagonist gene（IL-1rn）POLYMORPHISMS AND SUSCEPTIBILITY TO FEBRILE SEIZURES（FS）.Methods Matching case-control study was carried out,blood samples from 52 patients with FS and 53 healthy children were collected.Genomic DNA was extracted and examined by polymerase chain reaction（PCR）amplification for judging IL-1RN genetype.Results Only Ⅰ/Ⅰ,Ⅰ/Ⅱ,Ⅱ/Ⅳgenetypes of IL-1RN were found in the surveyed Chinese population of Han nationality in Guangdong Province.No significant differences of these genotypes were observed in patients and controls.But the allele frequencise of Ⅰ,Ⅱand Ⅳ in patients were 95.2%,3.8% and 1.0%,respectively,while these frequencise in controls were 84.9%,10.4%and 4.7%,respectively.The allele frequency of IL-1RN Ⅰ in patients was obviously higher than that in controls（P〈0.05）.Conclusion The allele ⅠIL-1RN may be associated with susceptibility of FS.

关 键 词：白细胞介素1受体拮抗剂基因 基因多态性 惊厥 发热性 

分 类 号：R720.597[医药卫生—急诊医学]