川芎嗪注射液对生理状态下大鼠心室组织一氧化氮分泌的影响  

Effects of Ligustrazine on Secretion of Nitric Oxide in Ventricular Tissues of Rats under Physiological Condition

作　　者：张前进[1] 王燕飞[2] 杜军保[2] 

机构地区：[1]北京大学第一医院中医科,北京100034 [2]北京大学第一医院儿科,北京100034

出　　处：《实用儿科临床杂志》2007年第7期528-529,共2页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(30571971)

摘　　要：目的探讨川芎嗪注射液对生理状态下大鼠心室组织一氧化氮(NO)分泌的影响。方法将正常Wistar大鼠分为对照组和川芎嗪15mg/L组、川芎嗪150mg/L组,每组8例。分别将其左心室和右心室的组织剪碎,用Krebs液作为孵育液,放入37℃恒温水浴箱中振荡孵育4h,孵育后,用分光光度法测定孵育液中NO2-/NO3-。结果与对照组比较,左心室川芎嗪150mg/L组可NO释放减少(P<0.01),而川芎嗪15mg/L组与对照组比较无差异。右心室川芎嗪150mg/L组可促进NO释放(P<0.01),而川芎嗪15mg/L组与对照组比较无差异。结论在生理状态下,川芎嗪可抑制大鼠左心室组织NO释放,促进大鼠右心室组织中NO的释放。Objective To explore the effects of ligustrazine on secretion of nitric oxide（NO） in ventricular tissues of rats under physiological condition. Methods Twenty -four normal wistar rats were divided into three groups,eight each group. The left and right ventricular tissues of each rats were divided into control group and 2 ligustrazine groups and the final concentrations of ligustrazine were 15 mg/L and 150 mg/L respectively. The ventricular tissues that had been cutting into small pieces were incubated in 37℃ thermostatic water bath for 4 hours with Krebs solution as supernatant. After incubation of the ventricular tissues, the spectrophotometric method was used to detect the content of NO2 ^-/NO3^ - in the supernatant. Results Compared with the content of NO in control group,it was significantly decreased in the 150 mg/L ligustrazine group（ P 〈 0.01 ） in the left ventricular tissues, however, there was no significant difference between the 15 mg/L ligustrazine group and control group. Compared with the content of NO2 ^-/NO3 ^- in the control group,it was significantly increased in the 150 mg/L ligustrazine group（ P 〈 0.01 ） in the right ventricular tissues, however, there was no significant difference in content of NO2^ -/NO3 ^- between the 15 mg/L ligustrazine group and control group. Conclusion Under physiological condition, ligustrazine can inhibit the release of NO in the left ventrieular tissues of rats,while promote the release of NO in the right ventricular tissues of rats.

关 键 词：川芎嗪 一氧化氮 心室 组织孵育 

分 类 号：R285.5[医药卫生—中药学]