TNF-α对3T3-L1脂肪细胞PPAR-γ2 mRNA表达及脂联素分泌的影响  

Effects of TNF-αon PPAR-γ2 mRNA expression and adiponectin secretion in 3T3-L1 adipocytes

作　　者：邓大同[1] 王佑民[1] 刘玲[1] 胡国平[1] 杨明功[1] 佘其美[1] 王长江[1] 

机构地区：[1]安徽医科大学内分泌代谢病研究所安徽医科大学第一附属医院内分泌科,230032

出　　处：《中华内分泌代谢杂志》2007年第2期168-169,共2页Chinese Journal of Endocrinology and Metabolism

基　　金：安徽省自然科学基金资助项目(03043719);安徽省卫生厅科研基金(2002A008)

摘　　要：用肿瘤坏死因子α(TNF-α)分别处理未分化、已分化的3T3-L1细胞,检测培养细胞过氧化物酶体增殖物激活受体γ2(PPAR-γ2)mRNA的表达和脂联素的分泌。结果表明TNF-α可明显抑制3T3- L1脂肪细胞的PPAR-γ2 mRNA表达和脂联素的分泌(P<0.05或P<0.01),提示TNF-α可能通过PPAR-γ影响脂联素的分泌。Undifferentiated and differentiated 3T3-L1 adipocytes were treated with 100 ng/ml tumor necrosis factor-or （TNF-α）, and peroxisome proliferator-activated receptor-γ2 （PPAR-γ2） mRNA expression and adiponectin secretion in cultured ceils were measured. The results showed that TNF-α suppressed PPAR-γ2 mRNA expression and adiponectin secretion in 3T3-LI adipocytes （P 〈0.05 or P 〈0.01 ） , suggesting that TNF-α may affect adiponectin secretion via PPAR-γ.

关 键 词：脂联素 PPAR-Γ2 3T3-L1细胞 肿瘤坏死因子Α 

分 类 号：R587.1[医药卫生—内分泌]