高比活度[甲基一~3H]胸腺嘧啶核苷的制备  

Preparation of tritium labelled thymidine with high specific activity

作　　者：董墨[1] 曹蓉珍[1] 

机构地区：[1]中国科学院上海原子核研究所

出　　处：《核技术》1997年第1期49-52,共4页Nuclear Techniques

摘　　要：以５—甲酰基尿嘧啶为起始原料，采用“二步法”：第一步合成了氚标记的５—羟甲基尿嘧啶，第二步合成了氚标记的胸腺嘧啶。放射性比活度分别达到１．６ＰＢｑ／ｍｏｌ和２．２ＰＢｑ／ｍｏｌ，再经酶促反应，生成氚标记胸腺嘧啶核苷，其比活度达２．２ＰＢｑ／ｍｏｌ，放化纯度大于９５％Methy1 3H] thymine was synthesized from 5 formyluracil by the “two-stepmethod”.As the first step,5 [hydroxymethy1 3H] hydroxymethyluracil was prepared with specific radioactivity of 1.6PBq/mol.Then [methy1 3H]thymine was synthesizedvia enzymatic reaction with specific radioactivity of 2.2PBq/mol.The radiochemical purity of the product was over 95%.

关 键 词：氚标记 胸腺嘧啶核苷 同位素标记 

分 类 号：R817[医药卫生—影像医学与核医学]