病毒感染对栉孔扇贝血细胞类型和功能的影响  

The effect of spherical virus on populations and functions of haemocytes in Chlamys farreri

作　　者：许秀芹[1] 王宜艳[2] 孙虎山[2] 

机构地区：[1]上海水产大学生命科学与技术学院,上海200090 [2]鲁东大学生命科学学院,山东烟台264025

出　　处：《上海水产大学学报》2007年第3期242-247,共6页Journal of Shanghai Fisheries University

基　　金：国家"九七三"计划资助项目(G1999012005);鲁东大学校基金资助项目(20053303)

摘　　要：用从胶州湾大规模死亡栉孔扇贝体内分离出来的球状病毒注射感染栉孔扇贝(Chlamys farreri),分别于病毒感染后8 h、24 h、48 h、72 h和128 h取血,采用流式细胞仪技术研究了病毒对栉孔扇贝血细胞类型及死亡率的影响,采用荧光显微技术研究了病毒感染的栉孔扇贝血细胞的体外吞噬和包囊化作用的变化。结果表明,病毒感染的扇贝血细胞各类型的比例同对照组相比有显著的差异,在24 h、48 h和72 h,实验组透明细胞比例显著高于对照组,并呈逐渐升高趋势,在8 h、24 h、48 h、72 h,实验组大颗粒细胞比例显著低于对照组。在24 h、48 h、72 h和128 h,实验组血细胞的死亡率显著高于对照组。病毒感染后,实验组扇贝血细胞的体外吞噬率和包囊化率都显著高于对照组。Haemocyte characterization of Chlamys farreri was assayed in this study after injection of the spherical virus isolated from the infectious tissues of the scallop collected in the period of massive death in Jiaozhou Bay. At 8 h, 24 h, 48 h, 72 h and 128 h after injection with spherical virus, population proportion and mortality of haemocytes from C. farreri were assayed by flow cytometry, and effect of virus on haemocyte functions, including phagocytosis and encapsulation in vitro, was studied by fluorescence microscope. The result showed there was significant difference between subpopulation proportion of experimental groups and control. After infection with virus, the hyalinocyte proportion of haemocytes of experimental groups was significantly higher than that of control groups at 24 h, 48 h and 72 h. But the big granulocyte proportion was significantly lower than that of control at 8 h, 24 h, 48 h and 72 h. Mortality of haemocytes of experimental groups was significantly higher than that of control at 24 h, 48 h, 72 h and 128 h. The phagocytosis and encapsulation rate of haemocytes of experimental groups was significantly higher than that of control.

关 键 词：栉孔扇贝 球状病毒 血细胞 流式细胞术 

分 类 号：S944.3[农业科学—水产养殖]