嗜水气单胞菌重组β-hemA-ISCOMs对鳗鲡的浸泡免疫效果  

Immune effects of the eel after immersed with ISCOMs containing recombinant β-hemA of Aeromonas hydrophila

作　　者：吴宗福[1] 龚晖[1] 陈红燕[1] 杨金先[1] 刘晓东[1] 林天龙[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《水产学报》2007年第3期374-378,共5页Journal of Fisheries of China

基　　金：福建省自然基金项目(B0410025);福建省科技重大专项专题(2004NZ03-2);国家自然科学基金项目(30471336)

摘　　要：应用嗜水气单胞菌β-hemA重组菌表达产物作为抗原,制备成免疫刺激复合物(β-hemA-ISCOMs),通过浸泡途径免疫鳗鲡,测定免疫鱼细胞和抗体应答水平和免疫效力。试验结果表明:(1)β-hemA-ISCOMs在电镜下呈笼格状颗粒,直径约40nm;(2)一免后第44天,β-hemA-ISCOMs免疫组淋巴细胞转化率显著高于非β-hemA-ISCOMs免疫组(P<0.05);(3)免疫浓度在30～120mg.L-1时,β-hemA-ISCOMs免疫组血清抗体效价与浓度正相关,且显著高于ISM1312佐剂组与无佐剂组(P<0.05);(4)免疫浓度在30～120mg.L-1时,β-hemA-ISCOMs免疫组存活率与免疫接种剂量正相关,在同等剂量下,β-hemA-ISCOMs免疫组的存活率高于其它组。因此β-hemA-ISCOMs浸泡免疫可诱导宿主产生细胞与抗体介导的免疫应答,产生保护性免疫,ISCOMs技术适用于制备浸泡型渔用疫苗。The main objective of this work was to study the effect of β-hemA-ISCOMs in eliciting eel immunity. After immersed the eels with the different dose of vaccine , the resuls showed that： （ 1 ） β-hemA-ISCOMs appeared as a typical cage-like structure with the size about 40 nm in diameter; （2）The T lymphocyte proliferation of the fishes immunized with β-hemA-ISCOMs was significantly higher than the groups without ISCOMs as adjuvant（ P 〈 0.05）;（3） The value of serum antibody during β-hemA-ISCOMs groups is positively correlated with the dose of the vaccine at the range from 30 mg·L^-1 to 120 mg·L^-1. Furthermore, the serum antibody titer of β-hemA-ISCOMs group was significantly higher than ISM1312 adjuvant group and adjuvant free group; （4） The survival rates of β-hemA-ISCOMs groups was correlated with the dose of the vaccine at the range from 30 mg·L^-1 to 120mg·L^-1; at the same dose, the survival rates of β-hemA-ISCOMs groups was significantly higher than ISM1312 adjuvant group and adjuvant free group. mount immunity when administered by immersion; vaccine. The conclusions： β-hemA-ISCOMs was able to elicit eel to the ISCOMs technology is suitable for developing immersion

关 键 词：鳗鲡 嗜水气单胞菌 溶血素 免疫刺激复合物 浸泡免疫 淋巴细胞 抗体 

分 类 号：S942.5[农业科学—水产养殖]