尼罗罗非鱼与萨罗罗非鱼及其正反杂交后代的微卫星分析  

Microsatellite analysis of Oreochromis niloticus,Sarotherodon melanotheron and their reciprocal hybrids

作　　者：颜标[1] 李思发[1] 蔡完其[1] 

机构地区：[1]上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海200090

出　　处：《水产学报》2007年第3期411-415,共5页Journal of Fisheries of China

基　　金：国家"十五"攻关项目--尼罗罗非鱼选育技术(2001BA505B0513)

摘　　要：Using microsatellite markers with 10 pairs of primers,genetic structure of Oreochromis niloticus,Sarotherodon melanotheron and their hybrids were analyzed.The results suggested that:① Six pairs of primers showed a rich polymorphic,which can be used as genetic markers for the genetic diversity study of these tilapias.② The analysis based on the six pairs of primers showed that the average heterozygosity(H)ranged as:O.niloticus ♀× S.melanotheron♂(0.725),S.melanotheron ♀ × O.niloticus ♂(0.692),S.melanotheron(0.537),O.niloticus(0.485).The highest effective numbers of alleles(Ne)existed in O.niloticus♀×S.melanotheron♂(3.79),the lowest existed in S.melanotheron(1.92).The average polymorphism information content(PIC)of 4 tilapias was 0.584,the rank from high to low as,O.niloticus ♀ × S.melanotheron ♂(0.668),S.melanotheron ♀× O.niloticus ♂(0.643),O.niloticus(0.375),S.melanotheron(0.333).③ The fact of enhanced heterozigosity of hybrids suggested that there were the genetic basement of cross breeding in tilapia.Using microsatellite markers with 10 pairs of primers, genetic structure of Oreochromis niloticus, Sarotherodon melanotheron and their hybrids were analyzed. The results suggested that： O Six pairs of primers showed a rich polymorphic, which can be used as genetic markers for the genetic diversity study of these tilapias. ② The analysis based on the six pairs of primers showed that the average heterozygosity（H） ranged as： O. niloticus ♀×S . melanotheron♂ （0. 725）, S . melanotheron ♀×O . niloticus ♂ （0. 692）, S . melanotheron （0. 537）, O. niloticus （0. 485）. The highest effective numbers of alleles（Are） existed in O. niloticus ♀× S. melanotheron♂ （3.79）, the lowest existed in S. melanotheron （1.92）. The average polymorphism information content （PIC） of 4 tilapias was 0.584, the rank from high to low as, O. niloticus ♀× S. melartotheron♂ （0.668）,S. melanotheron ♀× O. niloticus ♂ （0.643）,0. niloticus （0.375）,S. melanotheron （0.333）. （3） The fact of enhanced heterozigosity of hybrids suggested that there were the genetic basement of cross breeding in tilapia.

关 键 词：尼罗罗非鱼 萨罗罗非鱼 杂交后代 基因组DNA 微卫星标记 

分 类 号：S917[农业科学—水产科学]