胞浆分裂阻滞微核法对山东“10·21”辐射事故病人受照射剂量的估算  

Biological dose estimation by cytokinesis-block micronucleus assay on the patients exposed to accidental radiation

作　　者：姚波[1] 蒋本荣[1] 贾蜀琼[1] 满秋红[1] 邱立娟[1] 艾辉胜[1] 

机构地区：[1]解放军第307医院血液科,北京100071

出　　处：《解放军医学杂志》2007年第4期293-295,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家863计划资助项目(2002AA216801)

摘　　要：目的对山东“10·21”辐射事故中2例严重受照射者进行淋巴细胞微核(MN)检测,并估算受照射剂量。方法用胞浆分裂阻滞微核(CBMN)法对2例患者(A和B)的外周血和骨髓样本分别进行MN检测。结果2例患者的外周血培养均未见双核淋巴细胞。患者A的骨髓培养所获双核细胞极少,依据双核淋巴细胞多少粗估剂量>20Gy。患者B的骨髓MN率为2.42个/细胞,剂量估计为8.7(8.0～9.4)Gy,与用染色体畸变分析、物理方法及ESR法所估算剂量接近,与临床表现基本一致。结论MN法简便快速,结果准确,是除染色体畸变分析之外又一种可靠的生物剂量计。Objective A biological dose estimation by cytokinesis-block micronucleus （CBMN） assay was performed on 2 patients exposed to accidental radiation. Method Micronucleus assay and dose estimation by CBMN in peripheral blood and bone marrow were performed in 2 patients （A and B） suffering from radiation sickness. Results No binucleated lymphocytes were observed in peripheral blood of 2 patients. The results of bone marrow examination showed that only one binucleated celt was discovered in patient A. The rough estimation of radiation dose of A was above 20Gy according to the degree of reduction of binucleated cell in our previous study. The micronueleus frequency was 2. 42 per cell and the dose estimation was 8.7 （8. 0-9. 4） Gy for patient B. These results were approximately in accordance with that of dose estimations by physical method, chromosome aberration analysis, ESR method, and clinical manifestations. Conclusion MN assay is simple, quick and accurate. It is a reliable biodosimetry in addition to chromosome aberration analysis.

关 键 词：辐射损伤 生物剂量估计 胞浆分裂阻滞微核法 

分 类 号：R818.04[医药卫生—放射医学]