雄激素对骨骼肌萎缩大鼠泛素连接酶的调控作用研究  

Modulating effect of androgen on ubiquitin ligases in a rat model of skeletal muscle atrophy

作　　者：尹会男[1] 柴家科[1] 张瑾[2] 韩翠华[2] 申传安[1] 吴焱秋[1] 陶白江[3] 刘东兴[3] 

机构地区：[1]解放军总医院第一附属医院烧伤研究所,北京100037 [2]解放军总医院第一附属医院核医学科,北京100037 [3]第二炮兵总医院烧伤科

出　　处：《解放军医学杂志》2007年第4期337-339,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助项目(30571919);北京市自然科学基金资助项目(7072077)

摘　　要：目的研究睾酮对地塞米松所致大鼠骨骼肌萎缩的治疗作用以及对泛素连接酶表达的影响。方法40只雌性SD大鼠随机均分为对照组、地塞米松组、睾酮组、睾酮+地塞米松组,留取血浆和腓肠肌标本,采用图像采集和处理系统测量肌纤维横断面面积(CSA),定量PCR检测基因表达,放射免疫法检测睾酮含量。结果地塞米松可使腓肠肌中两种泛素连接酶MAFbx和MuRF1的基因表达均明显增高(P<0.01或0.05),睾酮对这两种基因的表达均无明显作用,但可明显减轻地塞米松引起的肌纤维CSA减小(P<0.01)。结论睾酮可减轻地塞米松引起的大鼠骨骼肌萎缩,其治疗作用可能并不是通过调控泛素连接酶MAFbx与MuRF1来实现的。Objective To study the effect of testosterone on skeletal muscle atrophy of rats induced by dexamethasone, and to explore the relationship between androgen and ubiquitin ligase expression in skeletal muscle. Methods Forty female SD rats were randomly divided into four group： control group （CON）, dexamethasone group （DEX）, testosterone group （TES） and testosterone plus dexametha- sone group （TES＋DEX）. Blood samples were collected from the abdominal aorta and then centrifuged. The supernatant was collected and stored at 20℃. Gastrocnemius was removed and stored at 80℃. The cross-sectional area （CSA） of muscle fiber was determined by microscopic images of digitized muscle sections, using a computer-based imaging processing system. The expression of muscle atrophy F-box （MAFbx） and muscle RING finger-1 （MuRF1） mRNA in skeletal muscle was determined by real-time PCR. The expression of testosterone protein in serum was determined by immunoradiometry. Results Dexamethasone increased the expression of MAFbx and MuRF1 mRNA in skeletal muscle （P〈0.01 or P〈0. 05）. Testosterone showed no influence to the expression of MAFbx and MuRF1 mRNA in both CON and DEX groups, but may obviously attenuate the decrease of muscle fiber CSA caused by dexamethasone （P〈0. 01）. Conclusion Testosterone may attenuate muscle atrophy induced by dexamethasone in rats, but ubiquitin ligases, MAFbx and MuRF1 in skeletal muscle may not be involved in this process.

关 键 词：雄激素 糖皮质激素 肌肉 泛素 连接酶 

分 类 号：R644[医药卫生—外科学]