罗布麻及其易混品的DNA分子鉴定  

Molecular Identification of Apoacynum venetum and its Confusable Species

作　　者：彭雪梅[1] 张卫明[1] 王蔓丽[1] 陆长梅[1] 顾龚平[2] 

机构地区：[1]南京师范大学生命科学学院江苏省资源生物技术重点实验室,南京210097 [2]南京野生植物综合利用研究院,南京210042

出　　处：《植物研究》2007年第3期302-307,共6页Bulletin of Botanical Research

基　　金："十五"国家科技攻关基金资助(2004BA502B10);江苏省高新技术研究计划(BG2006318)

摘　　要：为从分子水平更准确地鉴别罗布麻及其易混品,本研究利用PCR产物直接测序法对罗布麻、大叶白麻和Apoacynum cannabinum的核基因组(rDNA)ITS区与叶绿体基因组(cpDNA)trnL内含子及trnL-F间隔区序列进行测序与比较。结果显示,罗布麻和大叶白麻ITS序列完全一致,与A.cannabinum在ITS1区有13个位点、在ITS2区有10个位点不同;在trnL内含子区及trnL-F间隔区,罗布麻和大叶白麻共有3个位点不同,罗布麻和A.cannabinum间共有23个位点、大叶白麻和A.cannabinum间共有20个位点不同。研究表明,依据rDNAITS区序列可鉴别A.cannabinum和国产“罗布麻”(罗布麻与大叶白麻);利用cpDNAtrnL内含子及trnL-F间隔区序列可鉴别罗布麻及其相似种。To identify Apoacynum venetum and its confusable species on the molecular level, rDNA ITS （ including ITS1, 5. 8 s and ITS2） and cpDNA trnL intron as well as trnL-F non-coding region from A. venetum and its confusable species were sequenced and analyzed in this study. Results showed that ITS sequences from all samples of A. venetum were the same as those from all samples of Poacynum hendersonii, but differed from that of A. cannabinum at 13 sites in ITS1 region and 10 sites in ITS2 region. In trnL intron and trnL-F non-coding region, A. venetum differed from P. hendersonii at 3 sites while from A. cannabinum at 23 sites; In addition, P. hendersonii was different from A. cannabinum at 20 sites. The results demonstrated that ITS sequence could be used for the identification of ＂Luobuma＂ of China from A. cannabinum, and sequences of trnL intron and trnL-F cpDNA could be used for the identification among A. venetum, P. hendersonii and A. cannabinum.

关 键 词：罗布麻 大叶白麻 A cannabinum ITS TRNL内含子 trnL-F非编码区 DNA分子鉴定 

分 类 号：Q949.32[生物学—植物学]