S-腺苷蛋氨酸对人肝星状细胞增殖和氧应激及转化生长因子β1表达的影响  

Effects of S-ademetionine on the proliferation, oxidative stress and TGF-β1 expression of human hepatic stellate cells

作　　者：刘梅[1] 陆伦根[1] 窦爱霞[1] 陈尉华[1] 郑瑞丹[2] 茅益民 曾民德[1] 房静远[1] 

机构地区：[1]上海交通大学医学院附属仁济医院消化内科上海市消化疾病研究所,200001 [2]福建漳州解放军第一七五医院肝病治疗中心

出　　处：《肝脏》2007年第2期99-102,共4页Chinese Hepatology

基　　金：上海市重点学科建设项目(Y0205);上海市科学技术委员会基金(054009618)资助

摘　　要：目的研究S-腺苷蛋氨酸(SAM)对人肝细胞和肝星状细胞(HSC)(LX-2细胞株)增殖和氧应激及转化生长因子(TGF)β1表达的影响。方法用不同浓度的SAM培养肝细胞和HSC,用MTT法检测SAM对肝细胞和HSC增殖的影响;用次氮基三乙酸铁(Fe-NTa)和SAM共同培养肝细胞和HSC,检测超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量;用不同浓度的SAM培养HSC,ELISA方法检测HSC表达TGF-β1状况。结果在一定浓度范围内,SAM可抑制HSC增殖,而对肝细胞增殖无影响;SAM可增加肝细胞和HSC的SOD活力,降低其MDA含量,同时可抑制HSC的TGF-β1表达。结论SAM可抑制HSC增殖,保护肝细胞和HSC免受氧应激损伤,抑制HSC分泌TGF-β1,显示其具有抑制HSC增殖、抗氧化和抗肝纤维化作用。Objective To investigate the effect of S-ademetionine（SAM）on the proliferation, oxidative stress and transforming growth factorβ1 （TGFβ1） expression of human hepatocytes and hepatic stellate cells （HSCs）（ LX-2cell line）. Methods Human hepatocytes and HSCs were incubated with various concentrations of SAM, and the effects of SAM on the proliferation of hepatocytes and HSCs was studied by 3 -（4,5 -dimethyhhiazol-2-yl） -2,5 - diphennylterazolium bromide （MTT） colorimetric assay. Human hepatocytes and HSCs were co-cultured with SAM and ferric nitrilotriacetic acid （Fe-NTa）, superoxide dismutase （SOD） activity and malondialdehyde （MDA） contents were detected. HSCs were incubated with various concentrations of SAM, the level of TGF-β1 was detected with ELISA. Results Within a given level range of SAM, the proliferation of HSC was significantly suppressed, but the proliferation of hepatocytes was not affected. SAM obviously increased the activity of SOD and decreased the contents of MDA in hepatocytes and HSCs, and inhibited the expression of TGFβ1 in HSCs. Conclusion SAM can inhibit the proliferation of HSC, protect hepatocytes and HSCs from oxidative stress, and inhibit the secretion of TGFβ1 in HSCs.

关 键 词：氧应激 增殖 S-腺苷蛋氨酸 肝星状细胞 转化生长因子Β1 

分 类 号：R96[医药卫生—药理学]