抗盐胡杨Na^+/H^+逆向转运蛋白基因PeNhaD1的功能  

Function of the Putative Na^+/H^+ Antiporter Gene PeNhaD1 from Salt-resistant Populus euphratica Oliv.

作　　者：吕萍萍[1] 胡军[2] 陈少良[1] 沈昕[1] 尹维波[2] 陈宇红[2] 孙勇如[2] 胡赞民[2] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083 [2]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《植物生理与分子生物学学报》2007年第2期173-178,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金重点项目(No.30430430);国家高技术研究和发展(863)计划项目(2006AA10Z131)资助~~

摘　　要：将胡杨Na+/H+逆向转运蛋白基因PeNhaD1,分别转入对盐敏感的缺失质膜和缺失液泡膜Na+/H+逆向转运蛋白基因的酵母突变菌株ANT3和GX1中。结果表明,在pH6.0、Na+浓度为80mmol/L(固体培养基)或400mmol/L(液体培养基)的条件下,转化具有目的基因的酵母ANT3具有更高的耐盐性,而将目的基因转化到突变株GX1时,却不能提高其耐盐性。实验结果说明PeNhaD1可能是通过编码质膜Na+/H+逆向转运蛋白而提高酵母的耐盐性的,推测其在胡杨耐盐机制中的作用可能是提高拒盐性。Yeast complementation experiments were carried out to define the possible function of PeNhaD1, a Na^＋/H^＋ antiporter gene from Populus euphratica Oliv., a salt resistant tree. PeNhaD1 was introduced to the Saccharomyces cerevisiae mutant strain ANT3 （△enal-4∷HIS3 Anhal∷LEU2）, which lacks the plasma membrane Na^＋/H^＋ antiporter gene ScNHA1 or GX 1 （△nhx 1∷TRP1）, which lacks tonoplast Na^＋/H^＋ antiporter gene ScNHX1 （Figs.1, 2）. Our results showed that PeNhaD1 rescued the normal growth of ANT3 in the presence of high salt （80 mmol/L NaCl on solid medium or 400 mmol/L in liquid medium, pH 6.0） （Figs.3, 5）, but not that of GX1 （Figs.4, 6）, suggesting that PeNhaD1 may play a role in salt tolerance of Populus euphratica by maintaining the capacity for salt exclusion under saline condition.

关 键 词：胡杨 NA^+/H^+逆向转运蛋白 基因 功能互补 

分 类 号：Q943[生物学—植物学]