山羊小腔卵泡体外长期培养系统的建立  

Long-term Culture of Goat Small Antral Follicles in Vitro

作　　者：李键[1] 王建辰[1] 朱建楚[2] 康靖全[2] 

机构地区：[1]西北农业大学动物医学系 [2]西北农业大学实验中心,陕西杨陵712100

出　　处：《动物医学进展》1997年第1期21-24,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金;编号:39470519

摘　　要：显微手术法剥离山羊2mm以下小腔卵泡,以单个卵泡方式进行体外培养。结果表明:山羊小腔卵泡在体外适宜条件下可培养6～25d,在培养过程中,卵泡可维持类固醇激素的分泌和卵母细胞发育。以基础培养液中添加1IU/ml FSH、1IU/ml LH和10ng/ml IGF—Ⅰ的培养体系最适宜山羊小腔卵泡的体外培养。Intact goat small antral follicles were dissected from ovaries by a microsurgical method and cultured individually in 24—well tissue culture plate. The results showed that, goat small antral follicles could survive 6～25 d and preserve steroidogenesis and oocyte development during the culture period. The culture system which contains 1 IU/ml FSH, 1 IU/ml LH and 10 ng/ml IGF-I in the basic medium was most suitable for the long-term culture of goat small antral follicles in vitro.

关 键 词：小腔卵泡 卵泡 体外培养系统 山羊 

分 类 号：S827.3[农业科学—畜牧学]