鳗菌宁1号中盐酸氟哌酸及盐酸林可霉素含量的测定  

The Method for the Determination of Norloxacin Hydrochlorid and Lincomycin Hydrochlorid in Manjunning I

作　　者：张瑞坤[1] 刘佩玉[1] 

机构地区：[1]江苏省兽药监察所

出　　处：《中国兽药杂志》1997年第1期27-28,共2页Chinese Journal of Veterinary Drug

摘　　要：利用盐酸林可霉素在紫外区域无吸收峰这一特点，直接用紫外分光光度法在２７３±１ｎｍ波长处测定盐酸氟哌酸的含量；用甲醇分离两组分后，用微生物测定法测定盐酸林可霉素的效价。实验结果，盐酸氟哌酸的回收率为１００．２１％，ＣＶ＝０．３８％；盐酸林可霉素的回收率为９９．２％，ＣＶ＝０．１８％。Ultra Violet Spectrophotometry has been applied successfully to the determination of Norloxacin hydrochlorid, depending on Lincomycin hydrochlorid having no absorption spectrum in the rang 200 to 400 nm. The absorbance was measured at the maximun at 273nm. The biological assay of antibiotics of Lincomycin hydrochlorid was accomplished after the separation from Norfloxacin hydrochlorid with methanl, the recovery of Norloxacin hydrochlorid is 100.2%, CV=0.38%; the recovery of Lincomycin hydrochlorid is 99.2%, CV=0.18%

关 键 词：盐酸林可霉素 盐酸氟哌酸 测定 鳗菌宁1号 

分 类 号：S859.796[农业科学—临床兽医学]