黑粒糯小麦材料的鉴定  

Identification of Black Kernel Waxy Wheat Material

作　　者：郑壮丽[1] 王成社[1] 杨进荣[1] 刘俊[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《麦类作物学报》2007年第3期433-437,共5页Journal of Triticeae Crops

基　　金：国家"863"项目(2001AA241031)

摘　　要：为了给新型小麦材料黑粒糯小麦的选育提供基础材料,采用改良碘染色法和聚丙烯酰胺凝胶电泳对全糯小麦品系204-1002与黑粒小麦品系204-001、203-3204、201-2138杂交选育得到的30个黑粒糯小麦高代材料进行了籽粒颜色、Wx蛋白亚基以及淀粉颜色反应的鉴定与分析,结果发现7个黑粒小麦品系(1004、1007、1008-3、1009-3、1018-2、1018-4、1021-1)均缺失3个Wx蛋白亚基,同时鉴定出了缺失1个或2个Wx蛋白亚基的黑粒部分糯小麦品系。这表明利用全糯小麦与黑粒小麦杂交能筛选出黑粒糯小麦新材料。To provide the basic and new materials to black waxy wheat breeders, we use the improved Iodine staining method and SDS-PAGE electrophoresis to identify the Wx protein subunits and to analyze the color of starch chemical reaction in the 30 advanced lines of black kernel waxy materials coming from crosses between whole waxy wheat line 204-1002 hybrid with black kernel wheat lines 204-001,203-3204 and 201-2138. We found that 7 black kernel wheat lines 1004.1007.1008-3,1009-3, 1018-2.1018-4.1021-1, were all lack of three Wx protein subunits. At the same time, we identified some partly waxy black kernel wheat lines lack of one or two Wx protein subunits. The results indicated that we could screen out new material of black kernel waxy wheat with the hybridization between whole waxy wheat and black kernel wheat.

关 键 词：黑粒糯小麦 Wx蛋白亚基 糯性类型 

分 类 号：S512.4[农业科学—作物学] S331