兔骨髓间充质干细胞Ⅱ型胶原表达的实验研究  

Experimental Study for CollagenⅡ Expression in Rabbit Mesenchymal Stem Cells after Transfection of Ad-TGF β1

作　　者：秦宏敏[1] 佟向阳[1] 许铁[1] 姚爱明[1] 陈孜[1] 冯斌[1] 刘凯[1] 

机构地区：[1]徐州医学院附属医院创伤骨科,江苏省徐州市221002

出　　处：《中国骨与关节损伤杂志》2007年第5期391-393,共3页Chinese Journal of Bone and Joint Injury

基　　金：江苏省卫生厅医学科技发展基金(Z200318)

摘　　要：目的探讨兔骨髓间充质干细胞表达Ⅱ型胶原的实验研究方法。方法利用β1转化生长因子腺病毒表达载体(Ad-TGFβ1)转染兔骨髓间充质干细胞(MSCs),观察转染后的Ⅱ型胶原表达。结果细胞转染后Ⅱ型胶原在免疫印记和免疫组化检测下均呈强阳性表达。结论Ad-TGFβ1转染兔骨髓MSCs可使其细胞内Ⅱ型胶原表达阳性。Objective To explore the collagen Ⅱ expression after transfection of rabbit marrow mesenchymal stem cells by ad - TGFβ1. Methods The Ad - TGFβ1 was transfected to the rabbit marrow MSCs and then the collagen Ⅱ expression was detected. Results The cells after transfection were detected by Western blot and immunohistochemical staining. The collagen was all positive. Conclusion The rabbit marrow MSCs transfected by Ad - TGFβ1 could express collagen Ⅱ .

关 键 词：骨髓间充质干细胞 转染 Ⅱ型胶原 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]