骨形态发生蛋白7在慢性HBV感染者肝组织中的表达  

Expression of BMP-7 in Liver Tissue of Patients with Chronic HBV Infection

作　　者：丁芹[1] 马会慧[1] 崇雨田[1] 杨绍基[1] 

机构地区：[1]中山大学附属第三医院感染科

出　　处：《中山大学学报（医学科学版）》2007年第3期279-283,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金资助项目(30571665)

摘　　要：【目的】通过对不同慢性乙型肝炎病毒(HBV)感染者肝组织中骨形态发生蛋白7(BMP-7)表达的分析,初步探讨BMP-7在肝脏炎症和肝细胞再生过程中可能发挥的作用。【方法】对56例慢性HBV感染者及5例正常对照肝组织进行常规组织学观察,按病理诊断分组。其中病毒性肝炎乙型慢性轻度12例,中度12例,重度6例,重型8例,乙肝肝硬化10例,肝癌8例。进行BMP-7的免疫组织化学染色,观察光镜下分布特点,取部分标本进行图像分析。取8例肝癌患者癌结节及3例癌旁组织进行实时荧光定量RT-PCR,检测BMP-7mRNA的表达。【结果】BMP-7阳性单位在正常人肝脏(7.27±2.88)、病毒性肝炎(乙型)慢性轻度(8.50±3.22)、中度(10.96±2.88)、重度(13.11±1.26)有逐渐增加趋势,重型肝炎(7.50±1.21)、肝硬化(8.24±1.79)较中度、重度低;进一步比较BMP-7阳性区灰度有相似结果(P值均小于0.01)。BMP-7在肝脏既有胞浆型表达,也有胞膜型表达。在慢性中度、重度患者及肝硬化结节内为弥漫性表达;但在重型肝炎患者,仅呈灶状表达;在癌周组织有弥漫或灶状表达,在癌结节表达极少。【结论】在慢性乙型肝炎患者肝组织中BMP-7表达增多,但是在重型肝炎肝组织及肝癌结节中表达减少,值得进一步研究。[Objective] To investigate the possible effects of bone morphogenetic protein 7 （BMP-7） on liver inflammation and hepatocyte regeneration by analysis of its expression in the liver of patients with chronic hepatitis B virus （HBV） infection. [ Methods ] Liver tissues of 56 patients were overviewed in histology among which there were 12 mild chronic hepatitis B, 12 moderate hepatitis B, 6 severe chronic hepatitis B, 10 hepatic cirrhosis,8 chronic hepatic failure and 8 hepatic carcinoma according to their pathologic diagnosis. Liver tissues of 5 normal liver donors were enrolled in this study as normal control. The feature of BMP-7 expression was studied by histochemical stain and quantitated BMP-7 expression of some specimen by image analysis. BMP-7 mRNA expression in 8 liver cancer tissues and 3 peri-neoplasma tissues were quantitated by real-time RT-PCR. [Results] BMP-7 expression positive units （PU） in normal control（7.27 ± 2.88）,mild chronic hepatitis B （8.50 ± 3.22）, moderate hepatitis B （10.96 ± 2.88） and severe chronic hepatitis B （13.11 ± 1.26） was noticed to increase gradually. The PU in hepatic cirrhosis （8.24 ± 1.79）or chronic hepatic failure （7.50 ± 1.21） was lower than in moderate or severe chronic hepatitis B. Similar consequences were seen after further comparison of the gray scale of BMP-7 positive domain. Investigation of the expression pattern showed that BMP-7 expressed both in the cytoplasm and cell membrane. The pattern in moderate and severe hepatitis or nodus of hepatic cirrhosis was diffused, while in chronic hepatic-failure, there were only some limited expression. Surprisingly, little positive expression could be found in carcinoma nodus, but was dramatically in peri-neoplasma tissues. [Conclusion] BMP-7 expression in the liver of chronic hepatitis B patients increased, but decreased in chronic hepatic failure or liver cancer. It deserves further investigation.

关 键 词：骨形态发生蛋白7 肝炎 肝硬化 肝癌 

分 类 号：R512.62[医药卫生—内科学]