基于基因组学的日本血吸虫序列分析平台的构建及应用  

Construction and Application of Sequence Analysis Platform Based on Genomics of Schistosoma japonicum

作　　者：刘稳升[1] 胡斐[2] 吕志跃[2] 郑焕钦[2] 袁衡新[1] 吴忠道[2] 

机构地区：[1]中山大学中山医学院生物医学工程系,广东广州510080 [2]中山大学中山医学院寄生虫学教研室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2007年第3期348-352,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金资助项目(NO.30271165);教育部博士基金资助项目[(2005)216号]

摘　　要：【目的】构建基于基因组学的日本血吸虫序列分析平台。【方法】基于工作站和Linux操作系统,利用公开的日本血吸虫或相关资源,收集有关生物信息学序列分析工具,并自行编写相关软件实现多种资源的有机整合。【结果】构建了本地化的日本血吸虫序列分析平台,在此基础上,自主设计了日本血吸虫EST序列的电子延伸系统、基于日本血吸虫EST数据库的同源全长cDNA序列检索系统和序列自动注释系统等。【结论】所构建的序列分析平台不仅可以完成常规的序列分析工作,还可成功地完成EST序列的电子延伸,同源全长cDNA序列的检索以及序列的自动注释,它可对大规模日本血吸虫序列进行快速、方便、高效的分析,为充分利用血吸虫基因组开展疫苗研究和新药开发提供了有效的技术手段。[Objective]To construct a sequence analysis platform for the sequences of Schistosoma japonicum based on genome. [ Methods ] Based on workstation and Linux operation system, using public resources of Schistosoma japonicum or related data, collecting some related bioinformatics tools for sequences analysis, and integrating organically some resources by programming at the mean time. [Results]A platform was constructed successfully. Founded on this platform, we have developed the in silico EST elongating system of Schistosoma japon/cum, homological full-cDNA sequence retrieval system based on EST database of Schistosoma japonicum, and auto sequence annotation system et al. [ Conclusion ]This system can not only complete routine jobs of sequences analysis, but also complete in silico EST elongating, homological full-cDNA sequence retrieval and auto sequence annotation successfully. It is proven that the sequence analysis platform is available for utilizing to analyze the sequence data in high-throughput, rapidly, conveniently and efficiently, and subsequently, and help throwing new light on vaccine candidates and drug targets for Schistosomiasis japonica.

关 键 词：日本血吸虫 基因组 序列分析系统 

分 类 号：R383.2[医药卫生—医学寄生虫学]