胸腺九肽对鸡脾淋巴细胞增殖转化及IL-2基因表达与分泌的影响  

Effects of thymulin on proliferation and differentiation of chicken splenic lymphocytes cultured in vitro and on IL-2 gene expression and secretion of IL-2

作　　者：王丙云[1] 梁敏[1] 计慧琴[1] 陈胜锋[1] 

机构地区：[1]佛山科学技术学院动物医学系,广东南海528231

出　　处：《中国兽医科学》2007年第5期425-428,共4页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30070557);广东省自然科学基金项目(501237)

摘　　要：采用MTT比色法、相对半定量RT-PCR法和放射免疫测定(RIA)法研究了不同浓度的胸腺九肽对离体培养的鸡脾淋巴细胞增殖转化I、L-2基因表达与分泌的影响。结果表明,用10 ng/mL和1 ng/mL胸腺九肽处理鸡脾淋巴细胞8 h后,能显著增加由ConA诱导的淋巴细胞增殖转化反应,显著提高IL-2基因的表达水平以及细胞培养上清液中的IL-2含量(P<0.05);100 ng/mL的胸腺九肽虽有增强脾淋巴细胞增殖转化反应以及提高IL-2基因表达和分泌能力的趋势,但与对照组无显著差异(P>0.05)。提示,胸腺九肽能增强鸡脾淋巴细胞的增殖转化反应,并主要在基因转录水平上促进IL-2的合成与分泌,其作用与剂量大小有关。Effects of different concentrations of thymulin on the differentiation and proliferation of chicken splenic lymphocytes and IL-2 gene expression and secretion of IL-2 were tested by MTT colorimetry,relative semi-quantitative RT-PCR and radio immunoassays （RIA）, respectively. The results showed that treatments with 10 ng/mL and 1 ng/mL of thymulin for 8 h promoted significantly the differentiation and proliferation of the cells induced with concanavalin（ConA） ,and the expression of IL-2 gene＇s mRNA. And 100 ng/mL of thymulin had no significant effects （P〉0.05） on the differentiation and transformation of the ConA-induced cells,and the expression of IL-2 gene. The results suggested that thymulin could enhance the differentiation and proliferation of the cells and mainly promote the synthesis and secretion of IL-2 on gene transcription level,which was dependent on concentration of thymulin.

关 键 词：胸腺九肽 鸡 淋巴细胞增殖 IL-2基因 

分 类 号：Q574[生物学—生物化学] Q786