人卵透明带蛋白原核表达载体的构建  

Construction of recombination human zona pellucida protein expression vector in prokaryotic cells

作　　者：郭焱[1] 邹冬辉[1] 路英丽[1] 赵慧[1] 李丹地[1] 王忠山[1] 

机构地区：[1]吉林大学基础医学院细胞生物教研室,130021

出　　处：《中国妇幼保健》2007年第13期1792-1794,共3页Maternal and Child Health Care of China

基　　金：国家自然科学基金资助项目(39870313)

摘　　要：目的:构建人卵透明带蛋白(huZP3)基因原核表达载体。方法:利用PCR法扩增出含人卵透明带蛋白编码基因,先将其克隆到pGEM-T载体,进行序列测定和分析,然后将huZP3蛋白编码基因亚克隆至原核表达载体pGEX4T-1上,酶切鉴定。结果:获得一个核苷酸长度约为1200bp的基因,同源比较结果表明,与GenBank(NCBI:M60504)公布的ZP3基因序列有100%的同源性。酶切表明ZP3基因正确插入原核表达载体pGEX4T-1。结论:成功构建出含ZP3基因的原核表达载体。Objective： To construct expression vector which can express human zona pellucida in prokaryotic cells. Methods： The ZP3 gene was amplified by PCR. The amplified DNA fragment was cloned into pGEM -T vector for sequencing analysis. The ZP3 gene was then subcloned into prokaryotic expression vector pGEX4T - 1. Results： A gene of 1200 bp was obtained and showed a 100% homology with ZP3 gene sequence published in GenBank （NCBI： M60504 ）. ZP3 gene was inserted into prokaryotic expression vector pGEX4T-1 correctly. Conclusion： ZP3 protion expression vector is constructed successfully.

关 键 词：人卵透明带蛋白 原核表达载体 构建 

分 类 号：R339.22[医药卫生—人体生理学]