RP-HPLC法测定复方银杏叶片中葛根素的含量  

Determination of Puerarin in Compound Ginkgo Biloba Tablets by RP-HPLC

作　　者：潘洪平[1] 李小燕[2] 危华玲[1] 蒙大平[1] 路玫[1] 

机构地区：[1]广西壮族自治区人民医院,南宁市530021 [2]广西民族大学,南宁市530006

出　　处：《中国药房》2007年第15期1161-1162,共2页China Pharmacy

基　　金：广西科学基金资助项目(桂科基0342003-1)

摘　　要：目的：建立以RP-HPLC法测定复方银杏叶片中葛根素含量的方法.方法：色谱柱为Kromasil C18（250mm×4.6mm,5μm）,流动相为甲醇-水（20∶80）,流速为1mL·min^-1,测定波长为250nm,柱温为35℃.结果：葛根素进样量在0.8～2.4 μg范围内与峰面积积分值呈良好的线性关系（r=0.999 3,n=5）;平均回收率为99.98%,RSD=1.31%（n=5）.结论：本法简便、灵敏度高、重现性好,可用于复方银杏叶片的质量控制.OBJECTIVE： To determine the content of Puerarin in Compound Ginkgo biloba tablets by RP - HPLC. METHODS： The determination was performed on Kromasil C18 column. The mobile phase consisted of methanol - water （20 ： 80） with a flow rate of 1mL· min^-1. The detection wavelength was 250 nm and the temperature of the column was 35℃ . RESULTS： The linear range of Puerarin was 0.8-2.4μg （r = 0.999 3, n = 5）, with the average recovery at 99.98%, RSD = 1.31% （n = 5） .CONCLUSION：The method is simple, sensitive and reproducible, and it can be used for the quality control of Compound Ginkgo biloba tablets.

关 键 词：复方银杏叶片 葛根素 反相高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]