采用PCR-CTPP检测白细胞介素2基因多态性  

Polymerase Chain Reaction With Confronting Two-Pair Primers(PCR-CTPP) For Geno Typing Interleukin 2 Polymorphism

作　　者：杨丽红[1] 陈必成[2] 潘晓东[2] 李澄棣[2] 昌盛 

机构地区：[1]温州医学院附属第一医院实验诊断中心,浙江温州325000 [2]温州医学院附属第一医院移植中心 [3]武汉同济医院

出　　处：《中国实验诊断学》2007年第5期569-571,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(30500468)

摘　　要：目的采用PCR-CTPP技术,检测中国浙南地区白细胞介素-2(IL-2)基因-330位的多态性,并分析其可能的临床应用。方法针对IL-2基因-330(T/G)多态性设计两条序列特异性引物,结合两侧引物,在同一反应管中进行PCR。对PCR条件进行优化,并与测序结果进行对比。在此优化条件对101名体检个体的DNA进行IL-2基因多态性分析。结果通过条件优化,PCR-CTPP(polymerase chain reaction with confronting two-pair primers)可以区分IL-2-330位T或G的单核苷酸的多态性,并与测序结果相符合。101位体检个体中TT纯合子为43例(42.6%),GG纯合子为11例(10.9%),T/G杂合子为47例(46.5%),结果按Hardy Weinberg分布(P>0.05)。结论PCR-CTPP可区分IL-2-330(T/G)多态性,且简单、方便,具有临床应用价值。Objective Interleukin 2 （IL-2）-330 （T/G） single nueleofide polymorphism （SNP） of south of Zhenjian province of China,were genotyped by a polymerase chain reaction （PCR） with confronting two-pair primers （PCR C＇TPP） .Methods Two allele-specific primers were designed and with other two flank primers to amplifieate DNA in one-tube PCR. Several PCR conditions were tested to establish the optimal conditions for distinguishing the allele-specifie bands for the polymorphism. Under the optimal PCR conditions, 101 Chinese health check-up examinees were genotyped. Results The allele-specific bands were successfully amplified under the optimal conditions of the PCR-CTPP, and the results were concordance with DNA sequencing.43 eases were T homozygote, 11 eases were G homozygote,47 cases were T/G homozygote. The genotype distributions were within the Hardy Weinberg equilibrium （ P 〉 0.05） .Conclusion IL-2-330 （T/G） SNP can be successfully genotyped by/：WAR CTPP, an inexpensive and time-saving genotyping tool, which will be useful to study on immunology.

关 键 词：IL-2 多态性 PCR-CIPP 

分 类 号：R392.1[医药卫生—免疫学]