G5型人A组轮状病毒LL36755株的VP4、VP6和NSP4编码基因的分子特征  

Molecular Characterization of VP4,VP6 and NSP4 Encoding Genes of G5 Human Group A Rotavirus LL36755 Strain

作　　者：李丹地[1] 段招军[1] 章青[1] 刘娜[1] 谢华萍[1] 崔淑贤[1] 靳淼[1] 杨学梅[1] 郑丽舒[1] 黄灿平[1] 匡治州[1] 王忠山[2] 方肇寅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052 [2]吉林大学基础医学院,吉林长春130021

出　　处：《病毒学报》2007年第3期195-201,共7页Chinese Journal of Virology

摘　　要：最近在亚洲首次发现并报道了感染人的G5型人A组轮状病毒LL36755株,为进一步探讨其进化来源,克隆了G5型人A组轮状病毒LL36755株的VP4、VP6、NSP4编码基因,并分析其基因序列的分子特征。结果发现卢龙株LL36755为罕见的G5P[6]型,其VP6的亚群为SGⅡ型,NSP4的基因型为B型。系统进化树分析表明,卢龙株LL36755的VP7、VP4编码基因与猪来源的毒株关系密切,而VP6、NSP4编码基因与人来源的毒株紧密相联系。可以推断新的人腹泻A组轮状病毒LL36755株是猪的VP7,VP4编码基因与人的VP6,NSP4编码基因的自然重组;而且该毒株不是G5的原型,很可能是人类轮状病毒与猪轮状病毒毒株的自然重组后逐步进化而来。Recently,we had discovered and reported the LL36755 in Asia. To further explore the evolutionary first time the human G5 group A rotavirus strain origin, the VP4, VP6, NSP4 encoding genes of the strain LL36755 were amplified by RT-PCR. Sequence analysis identified that LL36755 possessed the rare G/P combination of G5P[-6] , VP6 subgroup SG II and NSP4 genotype B. Rotavirus of G5P[-6] genotype was reported firstly in the world. Phylogenetic analysis revealed that VP7 , VP4 encoding genes of LL36755 was closely related to porcine strains, and VP6,NSP4 encoding genes related to human rotaviruses. There was perhaps an unusual combination between porcine VP7, VP4 and human VP6,NSP4 encoding genes . We hold that LL36755 strain is not the G5 prototype,and it probably derived from pig and human rotavirus strains naturally and evolved gradually after the reorganization.

关 键 词：人类轮状病毒 VP4 型 VP6 NSP4 重组 

分 类 号：R373.2[医药卫生—病原生物学]