肝细胞生长因子对辐射后心肌细胞蛋白合成的保护作用  

Hepatocyte growth factor enhances protein synthesis in cardiomyocytes exposed to gamma-ray irradiation

作　　者：胡舜英[1] 付朝平[1] 段海峰[2] 陈金龙[2] 王荣亮[2] 吴斌[2] 郭子宽[2] 陈国伟[3] 王立生[2] 

机构地区：[1]解放军第458医院内4科,广东广州510602 [2]军事医学科学院二所三室,北京100850 [3]中山大学第一附属医院心内科,广东广州510080

出　　处：《南方医科大学学报》2007年第5期602-604,共3页Journal of Southern Medical University

基　　金：国际原子能机构(IAEA)资助项目(13305/R1)~~

摘　　要：目的研究肝细胞生长因子(HGF)对60Coγ射线照射体外培养的大鼠心肌细胞蛋白表达的影响。方法体外培养新生大鼠心肌细胞,分为未照射组、单纯照射组、HGF处理照射组,照射组细胞分别用60Coγ射线20Gy单剂量照射心肌细胞,HGF处理的照射组在照射前3h用终浓度为40ng/ml的HGF孵育。在照射后48h:(1)用蛋白检测试剂盒检测各组心肌细胞中的总蛋白含量;(2)流式细胞仪检测各组的细胞周期变化;(3)带绿色荧光蛋白基因的腺病毒(AdGFP)感染各组心肌细胞,感染后48h用流式细胞仪检测各组心肌细胞中所含的绿色荧光蛋白的荧光强度,间接比较各组心肌细胞中绿色荧光蛋白的生成量。结果照射后48h,照射组心肌细胞中总蛋白含量较未照射组减低(P<0.01),HGF处理的照射组心肌细胞中总蛋白含量较单纯照射组增高(P<0.05)。照射后96h、感染AdGFP后48h,照射组心肌细胞的绿色荧光强度明显弱于未照射组(P<0.01),HGF处理的照射组心肌细胞的绿色荧光强度较单纯照射组增强(P<0.01)。结论60Coγ射线单剂量照射抑制体外培养的心肌细胞蛋白合成,HGF可部分逆转60Coγ射线对心肌细胞蛋白生成的抑制作用。Objective To investigate the protective effect of hepatocyte growth factor （HGF） on protein synthesis in rat cardiomyocytes exposed to gamma-ray irradiation. Methods Primary cultured cardiomyocytes were irradiated with single-dose （20 Gy） gamma ray in the absence or presence of HGF （40 ng/ml） added in the cell culture 3 h before the exposure. Forty-eight hours after irradiation, the total cellular protein was measured and cell cycle analyzed by flow cytometry. The cardiomyoctes were also infected with AdGFP 48 h after irradiation and the fluorescence intensity of the green fluorescence protein （GFP） in the cells determined by flow cytometry 48 h after infection. Results The protein synthesis was decreased significantly in the irradiated cardiomyocytes as compared with the control group （P〈0.01）, but was remedied significantly by incubation of the cells with HGF before the exposure （P〈0.05）. Flow cytometry revealed much lower mean fluorescence intensity （MFI） of GFP in irradiated cardiomycytes than in cells without the exposure （P〈0.01）; The MFI was higher in HGF-treated cardiomyocytes than in cells without HGF treatment following the exposure （P〈0.01）. Conclusion Gamma ray irradiation inhibits protein synthesis in cardiomyocytes, and HGF may attenuate this effect of gamma ray exposure for cardiomyocyte protection.

关 键 词：汗细胞生长因子 Γ射线 心肌细胞 蛋白合成 

分 类 号：R818.7[医药卫生—放射医学]