雷公藤内酯醇洗脱支架抑制支架内再狭窄及对PCNA、P27^(kip1)表达的影响  

Triptolide-eluting stent prevents porcine coronary artery in-stent restenosis by affecting PCNA and P27～(kip1) expression

作　　者：陆东风[1] 黄璟[1] 吴昊[2] 原萧[1] 张莉[1] 

机构地区：[1]广州医学院附属第二医院心内科,广东广州510260 [2]广东药学院附属第一医院ICU,广东广州510080

出　　处：《南方医科大学学报》2007年第5期667-670,共4页Journal of Southern Medical University

基　　金：广东省中医药局立项课题(103070);广东省科技厅立项课题(2005B33101012)~~

摘　　要：目的研究雷公藤内酯醇洗脱支架对冠脉支架植入术后再狭窄的有效性及安全性,及其对冠脉增殖细胞核抗原(PCNA)、P27kip1表达的影响。方法采用20只国内杂种幼猪,随机分成裸支架组和雷公藤内酯醇洗脱支架组,每组各植入支架10枚。比较支架植入后4周各组的冠脉造影、组织病理学、免疫组化及血生化结果。结果雷公藤内酯醇洗脱支架组支架内最小内径相似,大于裸支架组(P<0.05);雷公藤内酯醇洗脱支架组新生内膜面积小于裸支架(P<0.05)。雷公藤内酯醇洗脱支架组PCNA阳性细胞少于裸支架组(P<0.05),而P27kip1表达多于裸支架组(P<0.05)。未发现明显的毒副作用。结论雷公藤内酯醇能抑制新生内膜的形成,在支架植入后4周能预防再狭窄的形成,可能通过影响PCNA和P27kip1蛋白的表达抑制血管平滑肌细胞增殖发挥作用。Objective To investigate the effectiveness and safety of triptolide-eluting stents implanted in porcine coronary arteries for restenosis prevention, and its effect on the expression of proliferating cell nuclear antigen （PCNA） and P27^ktpl. Methods Ten triptolide-eluting stentts and l0 stainless steel stentts （control） were implanted in 20 porcine coronary arteries at random. Four weeks later, angiography of the arteries was performed along with also histopathological and immunochemical examinations. Results The in-stent minimal lumen diameter oftriptolide group was significantly greater, and the neointimal area significantly smaller, than those of the control group （P〈0.05）. PCNA expression was significantly lower while P27^ktpl protein significantly higher in triptolide group than in the control group （P〈0.05）. Conclusion Triptolide-eluting stent can effectively inhibit neointimal formation to prevent restenosis in porcine coronary artery 4 weeks after implantation, probably by inhibiting P27^ktpl expression and consequently vascular smooth muscle cell proliferation.

关 键 词：冠状动脉再狭窄 药物洗脱支架 雷公藤内酯醇 增殖细胞核抗原 P27 

分 类 号：R285[医药卫生—中药学]