激光打孔制备微孔猪脱细胞真皮基质  

Application of Laser Punching in Processing Porcine Acellular Dermal Matrix:a Preliminary Study

作　　者：梁黎明[1] 柴家科[1] 冯瑞[1] 李江明[2] 孙强[1] 尹会男[1] 

机构地区：[1]解放军总医院304临床部全军烧伤研究所,北京市100037 [2]华北光电技术研究所

出　　处：《中国激光医学杂志》2007年第2期88-91,共4页Chinese Journal of Laser Medicine & Surgery

基　　金：全军"十一五"医药卫生科研基金资助项目(06Z054);北京市科技计划课题资助项目(H060920050830);首都医学发展科研基金资助项目(2005-2040);解放军总医院科技创新课题资助项目(06ZY29)

摘　　要：目的探讨激光打孔制备微孔猪脱细胞真皮基质(PADM)的可行性。方法采用胰蛋白酶/Triton X-100的方法制备PADM,使用CO2激光加工机进行打孔。对比激光微孔PADM与传统网状PADM的外观。将48只SD大鼠背部制作全层皮肤缺损创面,随机分为3组,每组16只。微孔组:移植激光微孔PADM+自体刃厚皮;网状组:移植网状PADM+自体刃厚皮;对照组:仅移植自体刃厚皮。术后定期观察各组大鼠创面愈合情况,计算移植物成活率和创面收缩率。结果激光微孔PADM微孔呈矩阵样整齐排列,与网状PADM比较真皮基质分布更均匀。术后6周,微孔组移植物成活率与对照组比较差异无统计学意义,但明显高于网状组(P<0.05);微孔组创面收缩率明显低于网状组和对照组(P<0.05)。结论激光打孔可制备激光微孔PADM,其真皮基质与自体刃厚皮复合移植可提高创面修复质量。Objective To investigate the feasibility of processing porcine acellular dermal matrix （PADM） with the aid of laser punching technology. Methods PADM was prepared by the trypsin/Triton X-100 method. Micropores were produced on the PADM with a CO2 laser punch. The gross observation of the laser micropore PADM was compared with the traditional meshed PADbl. Full thickness defect wounds were created on the back of 48 SD rats. The rats were randomly divided into 3 groups： micropore group was grafted with laser mieorpore PADM and thin split-thickness autograft; mesh group was grafted with meshed PADM and thin split - thickness autograft; control group received simple thin split - thickness autografting. The gross observation of wound healing was performed at regular time points after surgery. The wound healing rate and contraction rate were calculated. Results Laser micropore PADM was characterized by its micropores arranged in arrays. The distribution of dermal matrix in laser mi- cropore PADM was more uniform than that in meshed PADM. Six weeks after grafting, the wound healing rate in micropore group was not statistically different from that in control group, but was significantly higher than that in mesh group （ P 〈 0. 05 ）. The wound con- traction rate in micropore group was remarkably lower than that in mesh group and control group （P 〈0. 05 ）. Conclusions Laser micropore PADM can be prepared with the aid of laser punching technology, and it can play an important role in improving the wound healing quality when cografted with thin split-thickness autograft.

关 键 词：脱细胞真皮基质 激光 微孔 皮肤移植 

分 类 号：TN249[电子电信—物理电子学]