抑制性消减杂交技术及其在动物基因研究中的应用  

Progress on the Suppression Subtractive Hybridization Technique and its Application in Animal Gene

作　　者：陶金忠[1] 张勇[1] 杨永新[1] 张进隆[1] 赵兴绪[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《动物医学进展》2007年第5期57-60,共4页Progress In Veterinary Medicine

摘　　要：抑制性消减杂交(SSH)是结合抑制性PCR和消减杂交技术发展起来的一种高效分离差异表达基因的方法,具有特异性高、假阳性率低、操作简便、灵敏度高等特点。在两种状态下基因的表达变化、差异基因的克隆和鉴定对基因表达调控有着重要意义。SSH在动物抗病、抗应激及药物的靶基因等方面的研究中取得了重要的进展。Suppression subtractive hybridization （SSH）is a technique of high efficiency for isolation differential expression gene at transcriptional level ,which is based on the suppression PCR and subtractive hybridization. Because of its characteristics of high efficiency,stability and reliability, SSH is widely used to study on resisting disease and resisting stress, target gene for medication. The methodology of SSH and its improvement, application on animal genes were reviewed.

关 键 词：抑制性消减杂交 聚合酶链反应 基因 动物 

分 类 号：S821.32[农业科学—畜牧学]