多拉菌素在犬体内药代动力学研究  

Pharmacokinetics of Doramectin in Dogs

作　　者：张萍[1] 王加才[1] 江善祥[1] 

机构地区：[1]南京农业大学动物医学院药理研究室,江苏南京210095

出　　处：《云南农业大学学报》2007年第3期389-392,共4页Journal of Yunnan Agricultural University

摘　　要：采用RP-HPLC法荧光监测器测定单剂量皮下注射多拉菌素[300μg/(kg BW)])后,犬体内不同时间点的血浆药物浓度。血浆药物浓度-时间数据采用3P97药动软件分析。结果表明,本试验条件下血浆中药物添加浓度在0.1-100 ng/mL范围内与峰面积呈良好线性关系,检测限为0.5 ng/mL。不同浓度水平的日内日间变异系数分别小于3%和4%,各浓度的平均回收率均大于90%。给药后血药浓度-时间数据符合一级吸收一室开放模型,主要药物动力学参数为:t1/2ka为(0.58±0.13)d,t1/2β为(4.78±0.31)d,Tmax为(2.00±0.29)d,Cmax为(20.29±2.04)ng/mL,AUC为(187.43±24.99)(ng.d)/mL。提示多拉菌素在犬体内血药浓度维持的时间长,消除缓慢,且根据其半衰期判断,多拉菌素属于慢性消除药物。The pharmacokinetics studies of doramectin in dog following single subcutaneous administration at a dose of 300 μg/kg were conducted in this paper. The concentration of doramectin was detected with reversed phase high performance liquid chromatorgraphic method with fluorescence detector. The concentration-time data were calculated with 3P97. Under this experiment condition, Lineary was expressed by correlation between chromatographic peak areas and concentration of standards at a wide concentration range of 0. 1 - 100 ng/mL, the standard calibration curve was linear in this range and the detectability was 0. 5 ng/mL. Three concentradtion levels of doramectin were analysed ,the intra-day and the inter-day coefficient of variance was respectively less than 3% and 4%, the average recovery was respectively more than 90%. The procession of doramectin after single subcutaneous administration was fit the one-compartment open model with first-order absorption. The main pharmacokinetic parameters of doramectin in dog were as follows： t1/2ka （0. 58±0. 13）d, t1/2β（4. 78 ±0. 31 ）d, Tmax （2. 00 ±0. 29）d,Cmax （20. 29 ± 2. 04） ng/mL, AUC （ 187. 43 ± 24. 99 ） （ ng · d）/mL. The results showed that doramectin has a long persistence in the plasma concentrations and eliminated slowly in dog. Based on the elimination halflife of doramectin in dog,it can be considered as a drug with slowly elimination rate.

关 键 词：犬 多拉菌素 高效液相色谱法 药动学 

分 类 号：S943.223[农业科学—水产养殖]