胶束电动色谱法和微柱液相色谱法分析白藜芦醇的比较  

Comparison of Micellar Electrokinetic Chromatography and Microcolumn High Performance Liquid Chromatography for Resveratrol Analysis

作　　者：范恩国[1] 黄晓晶[2] 张锴[1] 白玉华[1] 高如瑜[1] 阎超[2] 

机构地区：[1]南开大学生命科学院 [2]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《色谱》2007年第3期319-321,共3页Chinese Journal of Chromatography

基　　金：上海浦东新区科技发展基金企业博士后专项资金资助项目

摘　　要：白藜芦醇可以顺式和反式两种形式存在。它是一种具有药理活性的植物抗毒素,具有抗肿瘤、保护心血管、防止机体老化的功效。分别采用胶束电动色谱和微柱液相色谱两种方法对白藜芦醇的两种异构体进行了分离,并对这两种方法进行了比较。结果表明,两种方法均具有分离快速、重现性好、溶剂消耗少的特点,都适用于白藜芦醇的分析。Resveratrol, occurring in trans- and cis-isomeric forms, is a phytoalexin with pharmacological activities, for example, anti-cancer, anti-cardiovascular disease, and life-span increasing properties. Two analytical methods, namely, micellar electrokinetic chromatography （MEKC） and microcolumn high performance liquid chromatography （u-HPLC）, were developed and compared for resveratrol isomer separation. Both methods are suitable for resveratrol analysis owing to their performances of high speed, good reproducibility, and low consumption of solvents and samples.

关 键 词：微柱液相色谱 胶束电动色谱 白藜芦醇 异构体 方法比较 

分 类 号：O658[理学—分析化学]