功能失调性子宫出血子宫内膜组织中性激素及其受体表达变化的研究  

Study on Sex Steroid Hormones and Steroid Receptors in Endometria of Women with Adult Dysfunctional Uterine Bleeding

作　　者：康燕华[1] 张彦芳[1] 任雁林[1] 舒丽莎[1] 李建平[1] 常丽锦[1] 

机构地区：[1]河北北方学院附属第一医院妇产科,河北张家口075000

出　　处：《河北北方学院学报（医学版）》2007年第2期15-17,共3页Journal of Hebei North University:Medical Edition

基　　金：河北省科学技术研究与发展指导计划项目(编号:062761611)

摘　　要：目的：研究育龄期功能失调性子宫出血女性子宫内膜组织中性激素及其受体的变化。方法：随机采集2005．8-2006．11月河北北方学院附属第一医院妇产科门诊35例育龄期功血患者的静脉血及子宫内膜功能层组织，以正常月经健康女性20例排卵后期设为对照组。①采用放免法测定功血组及对照组女性血清及子宫内膜组织中雌二醇（E2）、孕激素（P）的含量；②HE染色后对子宫内膜进行组织学诊断；③用免疫组化法检测子宫内膜中雌、孕激素受体（ER、PR）的表达。结果：育龄期功血女性以排卵型为主（30／35），其子宫内膜组织中E2含量高于对照组，有统计学意义（P〈0．05），而P含量低于对照组（排卵后期的子宫内膜），有统计学意义（P〈0．05）；两组血清中E2、P含量无统计学差异（P均〉0．05），但血清、子宫内膜中E2和P呈正相关（rE2=0．74 PE2〈0．01，rP=0．65 Pp〈0．01）。结论：育龄期功血绝大多数可能是由于体内作用于子宫内膜的性激素产生的时间、数量及其受体比例失衡所致。Objective： To study the sex steroid hormones levels, the expressions of estrogen receptor （ER） and progesterone receptor（PR）in endometrial functional of women with adult dysfunctional uterine bleeding（DUB）. Methods.. The venous blood and endometrial functionalis samples were collected from 35 adult DUB cases and 20 healthy women with regular menstrual cycle as controls. ①The levels of E2, P in serum and endometrial tissue were assayed by immunoradiometry ② Endometrium histology after hematoryline and eosin （HE） staining was performed to identify as proliferative phase or secretory phase;③ The expression of ER. PR in endometrium were determined by immunohistochemistry. Results.. The majority of women with adult dysfunctional uterine bleeding ovalated. The mean E2 concentration of endometrial tissue was significantly higher in DUB patients than that in controls（P〈0.05） ;The mean P concentration of endometrial tissue was significantly lower in DUB cases than that in controls（P〈0.05）;whereas the mean serum E2 or P concentrations between two groups were not significant（both P〉0. 05）;Serum um tissue E2. P（rE2 =0. 74, PE2〈0. 01,rp=0.65,P〈0.01） Conclusion.. Adult dysfunctional Uterine Bleeding is probably linked to sex steroid phase window,amount and ratio disorder of steroid receptor in local endometria.

关 键 词：功能失调性子宫出血(DUB) 性激素 ER PR 

分 类 号：R711.52[医药卫生—妇产科学]