香蜂花组培快繁试验  

Experiment on axillary bud's induction and fast propagation of Melissa officnalis

作　　者：贾文庆[1] 刘会超[1] 陈韵[1] 

机构地区：[1]河南科技学院园林学院,河南新乡453003

出　　处：《广东农业科学》2007年第5期36-38,共3页Guangdong Agricultural Sciences

基　　金：河南省创新人才工程项目;河南科技学院重点项目基金(0546)

摘　　要：以香蜂花茎段为外植体进行组培快繁试验,结果表明,适合香蜂花茎节的消毒方式为70%酒精浸摇30 s后再用0.1%HgCl2浸泡7 min;适宜诱导分化香蜂花不定芽的培养基为MS+6-BA 2.5 mg/L+NAA 0.2 mg/L,诱导率达96%、愈伤化程度较小;适宜诱导生根的培养基为1/2MS+IBA 2.0 mg/L,生根率为95%,根长可达2.2 cm,且有利于侧根的形成。Stem segments of Melissa offwnalis were taken as explants to study it＇s fast propagation technology. The results showed that dipping with 70% alcohol for 30 seconds and 0.1% HgCl2 for 7 minutes was suitable for the disinfection of stem segments. The optimal medium for differentiation and multiplication of axillary buds was MS＋6-BA 2.5 mg/L＋NAA 0.2 mg/L, the inducing ratio was 96%. The most effective medium for rooting was 1/2MS＋IBA 2.0 mg/L, the rooting ratio was 95% and the length of root could reach 2.2 centimeters long.

关 键 词：香蜂花 组织培养 快速繁殖 

分 类 号：S682.39[农业科学—观赏园艺]