富血小板血浆对犬骨髓间充质细胞矿化能力的影响  

Effects of platelet-rich plasma on mineral characteristics of bone marrow stromal cells

作　　者：潘永海[1] 李宁毅[2] 邱建忠[3] 金晓明[2] 陈立强[2] 刘相萍[4] 隋爱华[4] 

机构地区：[1]青岛大学医学院二附院,青岛市中心医院口腔颌面外科,青岛266042 [2]青岛大学医学院附属医院口腔颌面外科,青岛266003 [3]青岛大学医学院附属市立医院口腔颌面外科,青岛266031 [4]青岛大学医学院附属医院分子生物实验室,青岛266003

出　　处：《口腔医学》2007年第5期232-235,共4页Stomatology

基　　金：国家自然科学基金资助项目(30471899)

摘　　要：目的研究富血小板血浆(platelet-rich plasma,PRP)对犬骨髓间充质细胞(marrow stromal cells,MSCs)碱性磷酸酶(alka-line phosphatase,ALP)及矿化结节形成能力的影响。方法将不同浓度(10%、20%、30%)的PRP及重组人骨形成蛋白(rhBMP-2)加入体外培养的第3代MSCs中,对照组为无血清培养液,检测其对细胞ALP活性的影响;将第3代的MSCs在体外进行培养,实验组加入矿化液和PRP(10%或30%),对照组只加矿化液。4周后用茜素红及Von-Kossa染色检测矿化结节的形成。结果10%PRP和rhBMP-2能显著提高MSCs的ALP活性,其中10%PRP对体外矿化结节的形成,亦有一定程度的促进作用。结论PRP有促进MSCs向成骨细胞方向转化的趋势。Objective To investigate the effects of platelet-rich plasma（PRP） on the alkaline phosphatase（ALP） activity and mineralized nodule formation of dog＇s bone marrow stromal cells（MSCs）. Methods Various concentrations of PRP（0,10%, 20%, 30% ） and rhBMP-2 were added to the medium of cultured third passage MSCs. After 3,6,9,12 days of co-incubation, the ALP activity was measured. After the third passage MSCs was cultured for 4 weeks, mineralized solution and PRP（ 10% ,30% ） were added into the test group medium. Mineralized solution was added into the medium of the control group. At day 28,the formation of mineralized nodules was detected with Dahl McGec-Russll＇ s alizarin red stain and yon Kossa＇ s silver nitrate for calcium. Results PRP at the concentration of 10% could increase ALP activity; the 10%PRP had an optimal effect（ P 〈 0.05）.PRP could also induce nodule formation to some degree. Conclusion PRP has the tendency of inducing MSCs to differentiate into osteoblastic cells.

关 键 词：富血小板血浆 骨髓间充质细胞 碱性磷酸酶 矿化 犬 

分 类 号：R318.19[医药卫生—生物医学工程]