复合血管内皮祖细胞的组织工程骨修复兔桡骨缺损的实验研究  

The effect of tissue engineering bone with the endothelial progenitor cells on repairing segmental defects of rabbit radius

作　　者：吴雪晖[1] 谢肇[1] 曾玲[1] 许建中[1] 罗飞[1] 陈卫军[2] 

机构地区：[1]第三军医大学西南医院骨科,重庆400038 [2]第三军医大学解剖学教研室

出　　处：《中华创伤骨科杂志》2007年第5期460-464,共5页Chinese Journal of Orthopaedic Trauma

基　　金：国家高技术研究发展计划资助重大攻关课题（863计划）（2006AA02A122）

摘　　要：目的评价复合血管内皮祖细胞（EPCs）的组织工程骨修复兔桡骨大段缺损的效果。方法选用68只新西兰大耳白兔，随机分为三组。制成桡骨中段15mm骨缺损模型，各组植入不同材料，实验组（EPCs组）：植入EPCs＋经成骨诱导的骨髓基质干细胞（BMSCs）＋脱钙骨基质（DBM）；对照组：植入BMSCs＋DBM；阴性对照组：单纯植入DBM。术后3d，2、4、8、12、16周行X线片、组织学光镜、透射电镜观察及12、16周生物力学测试。结果实验组的成骨活跃程度、骨愈合速度均明显优于对照组。12周实验组抗扭强度[（5．57±0．13）MPa]与正常对照组无差异[（5．44±0．26）MPa]，明显强于对照组[（4．72±0．58）MPa]，差异有统计学意义（P〈0．05）；16周实验组抗扭强度[（8．57±2．10）MPa]明显强于对照组[（5．43±0．22）MPa）1和正常对照组[（5．44±0．26）MPa）]，差异均有统计学意义（P〈0．05）；实验组与对照组各项指标明显优于阴性对照组，差异均有统计学意义（P〈0．05）。结论复合EPCs的组织工程骨骨诱导能力强，能促进骨愈合，是修复大段骨缺损的有效方法。Objective To evaluate the effects of tissue engineering bone with endothelial progenitor cells （EPCs） on the repair of segmental defects of rabbit radius, Methods Sixty-eight healthy New Zealand white rabbits were enrolled in the study and randomized into 3 groups, i.e. experimental group, control group and sham group. The rabbits in the experimental group were injected with EPCs and bone marrow stromal ceils （BMSCs） cultured on decalcified bone matrix （DBM）, while those in the control group were injected with BMSCs cultured on DBM, and those in the sham group were injected only with DBM. Models of segmental bone defect of 15 mm at the middle part of radius were established. X-ray analysis, histological and transmission electric microscope examinations were performed on postoperative day 3 and at postoperative weeks （POW） 2, 4, 8, 12 and 16. Biomechanical tests were carried out at POW 12 and 16. Results In the experimental group, the bone forming activity, bone healing speed and biomechanical strength were all significantly better than those in the control and sham groups（ P 〈 0. 05）. Conclusion Tissue engineering bone with EPCs may be effectively used to repair segmental bone defects, because it has strong bone inductivity and can accelerate bone healing process.

关 键 词：细胞 组织工程 桡骨骨折 

分 类 号：R686[医药卫生—骨科学]