超抗原单次和多次诱导T细胞膜表型及细胞因子释放的变化特征  

Expression characteristics of T cell surface molecules and cytokines induced by superantigen singly or repeatedly

作　　者：黄杨[1] 司少艳[1] 葛伟[1] 张秀敏[1] 胡沛臻[1] 李侠[1] 马斌[1] 隋延仿[1] 

机构地区：[1]第四军医大学基础部病理学教研室,陕西西安710033

出　　处：《医学研究生学报》2007年第5期455-458,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30271464)

摘　　要：目的:观察超抗原SEA体外单次和多次诱导T细胞的细胞膜表型及细胞因子释放的变化特征。方法:SEA体外单次和多次诱导小鼠脾淋巴细胞,用流式细胞术检测CD4、CD8、CD69、CD25、CD28及细胞膜上和细胞内CTLA-4(CD152)的表达,并测定细胞培养上清中IL-2、INFγ-、TNFα-、IL-4和IL-10的水平。结果:1次SEA诱导组(1×SEA组)中,CD4、CD8、CD69、CD25和CD28的表达均显著增加,CTLA-4的表达低,IL-2、INFγ-、TNF-α的水平显著增加。2×SEA组中,CD4、CD8、CD25和CD28的表达也有较明显的增高,CD69的表达未继续上升,CTLA-4的表达显著增加;IL-4和IL-10的水平明显增高。3×SEA组中,CD4和CD8的表达无明显增减,CD69、CD28和CTLA-4的表达显著降低,CD25的表达与2×SEA组相似;IL-10的水平仍继续上升。结论:SEA初次诱导小鼠脾淋巴细胞时,能显著促进T细胞的活化和增殖;经SEA多次刺激(3次)可诱导T细胞的无反应性。Objective ： To observe the expression characteristics of T cell surface molecules and cytokines induced by superantigen （SEA） singly or repeatedly in vitro. Methods：Mter single （1 × ） or repeated （n× ） induction by SEA in vitro, we examined CD4, CD8, CD69, CD25, CD28 and CTLA-4（CD152） expression on the T lymphocytes by FACS, and determined IL-2, INF-γ, TNF-α, IL-4 and IL-10 production in the supernant. Results：In the group of 1 ×SEA, the expression of CD4, CD8, CD69, CD25 and CD28 increased significantly, while the expression of CTLA-4 （CD152）was low, the production of IL-2, INF-γand TNF-α increased quickly. In the group of 2 × SEA, there were a little up-regulated expressions of CD4, CD8, CD25 and CD28, and significantly up-regulated expression of CTLA-4 and production of IL-4 and IL-10, whereas the expression of CD69 did not appear to change obviously. In the group of 3 × SEA, the expression of CD4, CD8 and CD25 did not increase or decrease obviously, and the expression of CD69, CD28 and CTLA-4 decreased significantly, the production of IL-10 remained up-regulated. Conclusion ：The activation and proliferation of naive T cells could be induced by SEA singly, and the T cells became anergic by repeated SEA induction in vitro.

关 键 词：超抗原 T细胞 细胞因子 细胞膜表型 

分 类 号：R392.11[医药卫生—免疫学]