乳腺癌患者腋下淋巴结来源的DC诱导自体特异性CTL的实验研究  

Induction of specific CTL by DC from axillary lymph node of patients with breast cancer

作　　者：刘运江[1] 张建立[1] 单保恩[2] 

机构地区：[1]河北医科大学第四医院外一科,河北石家庄050011 [2]河北医科大学第四医院科研中心,河北石家庄050011

出　　处：《细胞与分子免疫学杂志》2007年第1期60-62,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：河北省科技攻关课题基金资助(05276101D-66)

摘　　要：目的:研究乳腺癌患者腋下淋巴结单个核细胞来源的树突状细胞(DC)诱导抗原特异性细胞毒T淋巴细胞(CTL)的能力。方法:以多种细胞因子联合诱导腋下淋巴结单个核细胞中的贴壁细胞为DC,非贴壁细胞与IL-2共培养后诱导成为肿瘤区域引流淋巴结细胞(TDLNC),用自体乳腺癌细胞冻融抗原刺激DC,并和TDLNC共培养,以诱导肿瘤抗原特异性CTL。结果:淋巴结细胞经体外培养后,贴壁细胞在DC诱导前,其特异性表面标志物CD1a、CD83、CD86的百分比(%)分别为11.0±2.4、26.6±5.2和33.0±6.1,经与rhGM-CSF、rhIL-4共同培养,并经自体肿瘤冻融抗原加TNF-α诱导后3种标志物的水平升高,其百分比(%)分别为50.2±5.7、60.5±16.5和56.2±16.4(P<0.01)。TDLNC中CD3+和CD8+细胞含量(%)分别为73.9±2.2和32.8±3.2;经DC和肿瘤抗原诱导后,DC-Ag-TDLNC中的CD3+和CD8+细胞含量升高,其百分比(%)分别为82.7±2.8和62.5±2.5(P<0.01)。结论:乳腺癌患者腋下引流淋巴结中的单个核细胞在rhGM-CSF和rhIL-4刺激活化和自体肿瘤抗原及TNF-α的诱导下,可以分化为典型的DC。成熟的DC具有较强的抗原提呈功能,可以促进TDLNC增殖、分化为抗原特异性CTL。AIM： To investigate the capability of the dendritic cells （DC） from axillary draining lymph node of patients with breast cancer to induce the antigen specific CTL. METHODS： The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGM-CSF and rhIL-4 to induce DC. The non-adherent cells were cultured with IL-2 to induce tumor draining lymph node celIs（TDLNC）. DCs stimulated by the autoallergic breast cancer freeze-thawing antigen were cocultured with the obtained TDLNC to induce tumor antigen specific CTL. RESULTS： The positive percentage of CD1 a, CD83, CD86 on DC from freshly isolated mononuclear cells in axillary draining lymph node was 11.0 ± 2. 3, 26. 6 ± 5.2 and 33.0 ± 6. 1, respectively. After co-cultured with rhGM-CSF, rhIL-4, breast cancer freeze-thawing antigen and TNF-α, the percentage of CD1a, CD83, CD86 was increased to 50. 2 ± 5.7, 60. 5 ± 16. 5 and 56. 2 ± 16.4, respectively, （P〈0.01）. The percentage of CD8^＋ T cells in TDLNC increased from 32. 8 ± 3. 2 to 62. 5 ± 2. 5 after co-cultured with DC-Ag （P〈0.01）. CONCLUSION： Typical DCs can be induced from the mononuclear cells in axillary draining lymph node after stimulated with cytokine （rhGM-CSF, rhIL-4 and TNF-α）, which possess strong antigen presentation function and can stimulate TDLNC to proliferate and differentiate into antigen specific CTL.

关 键 词：乳腺癌 腋窝淋巴结 细胞因子 树突状细胞 特异性细胞毒T淋巴细胞 

分 类 号：R392.11[医药卫生—免疫学]