维生素A对高碘小鼠肝脏胰岛素样生长因子-I表达的影响  

Study on the influence of vitamin A on IGF-I concentration of iodine excess mice

作　　者：乔冬菊[1] 刘丽香[1] 申红梅[1] 于钧[1] 苏晓辉[1] 张智毅[1] 李颖[1] 张树彬[1] 赵馨[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心碘缺乏病研究所,150081

出　　处：《中国地方病学杂志》2007年第3期249-251,共3页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金项目（30671817）;黑龙江省自然科学基金（D0315）

摘　　要：目的观察维生素A（VA）对碘过量小鼠肝脏胰岛素样生长因子-I（IGF—I）表达的影响。方法昆明种小鼠按体质量随机分成6组：对照组（含碘50μg/L，VA4000U／kg）；高碘组（含碘3000μg/L，VA4000U／kg）；常碘低VA组（含碘50μg／L，VA0U／kg）；高碘低VA组（含碘3000μg／L，VA0U／kg）；高碘补VAI组（含碘3000μg／L，VA8000U／kg）；高碘补VA2组（含碘3000μg／L，VA16000U／kg）。喂养3、6个月时，ELISA法检测小鼠血清IGF—I水平，RT-PCR法检测肝脏IGF—I mRNA表达水平。结果与对照组相比，3个月时各组血清IGF—I水平未见明显改变．6个月时高碘组、常碘低VA组、高碘低VA组血清IGF—I水平明显下降（q=3．52、3．29、3．84，P〈0．05），高碘补VA组，IGF-I水平有所升高，介于对照组和高碘组之间。3、6个月时，高碘和常碘低VA组肝脏IGF-I mRNA表达水平，与对照组相比，均有降低趋势；3个月时高碘补VA组与高碘组比较，肝脏IGF—I mRNA表达有升高趋势，而6个月时，则未见明显改变，经检验各组差异均无统计学意义（P〉0．05）。结论高碘摄入抑制小鼠肝脏合成分泌IGF—I，补充VA可以部分拮抗高碘的抑制作用。Objective To observe the influence of vitamin A （VA） on liver Insulin like Growth Factors 1 （IGF-1） expression of iodine excess mice. Methods Kunming mice were randomly divided into six groups： normal （I- 50 μgg/L,VA 4000 U/kg）, HI（I- 3000 μgg/L,VA 4000 U/kg）, low VA（I- 50 μgg/L,VA 0 U/kg）, HI ＋ low VA（I- 3000 μgg/L,VA0 U/kg）, HI ＋ VAI （I- 3000 μgg/L,VA 8000 U/kg） and HI ＋ VA2（I- 3000 μgg/L,VA 16 000 U/kg） group. ELISA was used to measure the concentration of serum IGF-I and RT-PCR to measure the expression level of liver IGF-1 mRNA 3 and 6 months afterwards. Results In the 6th months, the serum IGF-I level of HI, low VA and HI ＋ low VA group decreased obviously compared with that of the normal group（q = 3.52,3.29,3.84,P 〈 0.05）, VA supplement increased the level of IGF-1 to some extent between normal and HI group. Liver IGF-1 mRNA expression level of HI and low VA group showed a decreasing tendency compared with that of the normal grouμg while it increased in HI ＋ VA groups in the period of the first 3 months, but kept unchanged later on. Conclusions The iodine excess inhibits the liver secreting IGF-1 in mice, which is partly antagonized by VA supplement.

关 键 词：碘 维生素A 胰岛素样生长因子-I 

分 类 号：R686[医药卫生—骨科学]