变应性鼻炎豚鼠鼻黏膜组织中一氧化氮含量与血流量的相关研究  

Nitric oxide content and blood flow in nasal mucosa of allergic rhinitis guinea pigs

作　　者：王素敏[1] 郑宏良[1] 章如新[1] 李兆基[1] 周水淼[1] 

机构地区：[1]第二军医大学长海医院耳鼻喉科,上海200433

出　　处：《第二军医大学学报》2007年第5期512-515,共4页Academic Journal of Second Military Medical University

基　　金：全军医药卫生科研基金(06MA164).~~

摘　　要：目的:观察豚鼠变应性鼻炎不同病程中鼻黏膜组织一氧化氮合酶(NOS)、一氧化氮(NO)含量及血流量的变化及其相互关系,进一步探讨变应性鼻炎的可能发病机制。方法:120只豚鼠随机分为正常对照组和致敏组,致敏组注射卵清蛋白制备变应性鼻炎豚鼠模型,正常对照组注射生理盐水替代卵清蛋白。分别干末次致敏前和末次致敏激发后即刻、激发后24、48、72 h处死豚鼠(n=6),取鼻黏膜组织,免疫组化染色测定其中内皮细胞型一氧化氮合酶(eNOS)和诱导型一氧化氮合酶(iNOS)的表达;采用NO检测试剂盒测定鼻黏膜组织NO含量;各组动物处死前检测鼻黏膜组织血流量。应用直线相关回归分析法分析豚鼠鼻黏膜组织中NO含量与血流量间的相关性。结果:激发前及激发后各时间点,致敏组豚鼠黏膜组织中iNOS的表达量明显高于正常对照组(P<0.01),而eNOS表达无显著差异。激发前致敏组豚鼠鼻黏膜组织中NO含量、鼻黏膜中血流量均明显高于正常对照组(P<0.01),激发即刻迅速下降至低于正常对照组(P<0.01),以后呈逐渐回升趋势,72 h基本接近激发前水平;而正常对照组各时间点基本无显著差异。致敏组豚鼠鼻黏膜组织中NO含量与鼻黏膜血流量之间有良好的线性关系(r=0.9935,P<0.001)。结论:变应性鼻炎豚鼠鼻黏膜组织iNOS蛋白过表达可能导致局部NO含量增多,从而增加局部鼻黏膜组织血流量。Objective：To examine the expression of nitric oxide synthase （NOS7 ,the content of nitric oxide and blood flow in nasal mucosa of allergic rhinitis guinea pigs, so as to further investigate the mechanism of allergic rhinitis. Methods： One hundred and twenty guinea pigs were randomly divided into control group （injected with normal saline） and allergic group （nasal challenge with egg albuminT. The guinea pigs were executed before and immediately, 24, 48, 72 h after the last nasal challenge; the expression of endothelial nitric oxide synthase （eNOS）and inducible nitric oxide synthase （iNOS） and the content of nitric oxide were examined in mucosa tissues. The blood flow in the nasal mucosa was determined in animals before execution. Linear regression correlation was used to analyze the relationship between the nitric oxide content and blood flow in nasal mucosa. Results： The immunostaining for iNOS in surface epithelium of allergic rhinitis guinea pigs was markedly stronger than that of normal guinea pigs at all time points （P〈0. 01） ; immunostaining of eNOS was similar in the 2 groups. Compared with those in normal guinea pigs, the content of NO and blood flow increased significantly before the last challenge with allergen （P〈0.01）, decreased significantly immediately after the last nasal challenge （P〈0. 01）, and then increased gradually and restored the normal level 72 h later; there was no significant difference in control group before and after challenge. The content of NO and blood flow in nasal mucosa was linearly correlated with each other in allergic group（P〈0. 01）. Conclusion： Overexpression of iNOS protein in the nasal mucosa of allergic guinea pigs may increase local NO content and subsequently increase the blood flow in local nasal mucosa.

关 键 词：鼻炎 变应性 常年性 鼻黏膜 血流量 一氧化氮 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]