百里酚蓝分光光度法测定阿昔洛韦及其反应机理  

Spectrophotometric Determination of Aciclovir with Thymol Blue and Its Reaction Mechanism

作　　者：秦宗会[1] 汪正文 谭蓉[3] 

机构地区：[1]涪陵师范学院化学及环境科学系,涪陵408003 [2]重庆武隆中学,武隆408500 [3]太极集团涪陵制药厂,涪陵408000

出　　处：《理化检验（化学分册）》2007年第5期354-357,359,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：重庆市第二批中青年骨干教师资助计划项目;涪陵师范学院科研项目(flsyk2006-25)资助

摘　　要：在pH7．84的NaH2PO4-Na2HPO4缓冲介质中，百里酚蓝与阿昔洛韦反应形成离子缔合物，最大吸收波长为596nm，并在436nm波长处产生负吸收。阿昔洛韦的浓度在2．05×10^-6～2．38×10^-5mol·L^-1内遵守比耳定律，ε=3．19×10^4L·mol^-1·cm^-1，检出限为6．21×10^-7mol·L^-1。若用双波长叠加，ε=4．46×10^4．·mol^-1·cm^-1，检出限为5．13×10^-7mol·L^-1。用于药品、血浆及尿液中阿昔洛韦的测定，RSD为0．68％～2．76％，回收率为99．1％～103．3％。对反应机理进行探讨，表明离子缔合物的形成不仅由静电引力引起，且与荷移作用和范德华力有关。In a phosphate buffer medium of pH 7. 84, an ion association complex was formed between aciclovir （ACV） and thymol blue （TMB） with its positive absorption maxima at 596 nrn, and negative absorption maxima at 436 nm. Linear relationships were obtained at both absorption maxima, and Beer＇s law was held in the range of 2. 05×10^-6 to 2. 38×10^-5mol · L^-1 of ACV at 596 nrn, with an apparent molar absorptivity （ε596） of 3. 19 ×10^4L· mol^-1 ·cm^-1, and a detective limit of 6. 21 × 10^-7 mol · L^-1. By applying the dual-wavelength measurement with wavelength addition method, the molar absorptivity was increased to 4. 46 ×10^4L· mol^-1 ·cm^-1 and a lower detection limit of 5. 13 × 10^-7 mol · L^-1 was obtained. Most of the commonly coexisting ions and substances were found not interfering. In the analysis of some a ciclovir drugs, and the RSD＇s （n = 6） calculated were in the range of 0. 68%-2. 76%. Results of recovery test obtained were in the range of 99. 1%-103. 3%. Reaction mechanism of the formation of ion association complex between ACV and TMB was studied and it was shown that the ion-association complex was formed not only due to electrostatic action, but also to charge transferring and to the van der waals force and the hydrophobic action.

关 键 词：分光光度法 百里酚蓝 阿昔洛韦 密度泛函理论 

分 类 号：O657.32[理学—分析化学]