水通道蛋白8在人羊膜上皮细胞WISH株的表达和定位  

Expression and localization of aquaporin 8 in human WISH cells

作　　者：李莉[1] 漆洪波[2] 

机构地区：[1]重庆市妇幼保健院,重庆400013 [2]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2007年第6期565-567,574,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(30400616)

摘　　要：目的:研究水通道蛋白8(AQP8)在人羊膜上皮细胞WISH株的表达和定位,羊水膜内吸收的分子机制。方法:培养人羊膜上皮细胞WISH株,采用Western blot技术检测WISH细胞AQP8蛋白表达,逆转录聚合酶链反应(RT-PCR)技术检测WISH细胞AQP8 mRNA的表达,免疫荧光组织化学方法检测WISH细胞上AQP8的分布。结果:WISH细胞能表达较低水平的AQP-8 mRNA,WISH细胞AQP8蛋白在34ku处有一明显的糖基化条带。在细胞内微粒体膜和细胞膜上均检测到标记AQP8的荧光。结论:人羊膜上皮细胞WISH有AQP8的蛋白和mRNA表达,AQP8可能介导了羊水膜内吸收。Objective：To determine the expression of aquaporin 8 and its localization in human WISH cells and to explore the molecular and cellular mechanisms for water absorption across the amniotic membranes. Methods： Human amnion-derive WISH cells were cuhured.Western analysis was used to quantify AQP8 expression level. Reverse transcripton-polymerase chain reaction （RT-PCR）was used to quantify AQP8 mRNA expression levels, lmmunofluorescence was used to determine localization of AQP8 in WISH cells. Resu/ts：AQP8 mRNA protein was found in human WISH cells. By western-blot,the AQP8 proteim was detected at 34ku in human WISH cells. AQP8 labeling was observed in intracellular vesicular structures throughout the cytosol and plasma membrane. Conclusion：The study demonstrates the expression of aquaporin 8 in human WISH cells.These results suggest that aquaporin 8 may be a channel that mediates amniotic fluid resorption by way of intramembranous pathway.

关 键 词：水通道蛋白8 WISH细胞 羊水调控 

分 类 号：R714[医药卫生—妇产科学]