休克血浆对豚鼠乳头状肌的电生理效应及益母草的干预作用  

Electrophysiological Effects of Shock Plasma on Guinea Pig Papillary Muscles and Inhibitory Effects of Leonurus heterophyllus Injetion

作　　者：马建伟[1] 刘文燕[2] 张晓云[1] 刘艳凯[1] 温晓竞[1] 焦宏[1] 王雪芳[1] 张利民[1] 

机构地区：[1]河北北方学院 [2]中国人民解放军93685部队医院

出　　处：《时珍国医国药》2007年第5期1047-1048,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省中医药管理局项目(No.05018;2006030)

摘　　要：目的探讨益母草对休克血浆所致豚鼠心室乳头肌电生理改变的拮抗作用。方法采用常规玻璃微电极细胞内记录技术,记录离体豚鼠心室乳头肌动作电位,观察休克血浆对豚鼠心室乳头肌动作电位的影响及益母草对其干预作用。结果正常血浆灌流时,与对照组相比,豚鼠心室乳头肌细胞动作电位无明显改变。休克血浆灌流时,豚鼠心室乳头肌细胞动作电位的APA,OS均显著升高,APD50,APD90,APD明显延长,与对照组及正常血浆组比较均有显著性差异(P<0.05);用不同浓度(2.5%,5%,10%)的益母草注射液灌流后,由SP引起的APA,OS升高出现明显降低,动作电位时程明显短于休克血浆组,尤其是APD90,APD缩短更为明显(P<0.01),此作用在浓度为10%时达到最强,且呈浓度依赖性。结论益母草注射液可明显拮抗休克血浆所致豚鼠心室乳头肌动作电位改变,对心肌具有一定保护作用。Objective To clarify the interential effects of Leonurus heterophyllus （LH） on shock plasma（SP） and protective effects on guinea pig papillary muscles. Methods By using conventional intracellular microelectrode technology to record their action potentials, LH were used to observe their electrophysiological effects on guinea pig papillary muscles. Results （ 1 ） In normal papillary muscles , shock plasma did not significantly increase the amplitude of action potential （APA） （P 〈 0.05 ）. （2）Perfusion with LH （2.5,5,10 % ）,partially abolished the effects of shock plasma on guinea pig and overshoot （OS） in a concentration in APD in papillary muscles. Conclusion SP can significantly influence eletrophysiological characteristics of the normal papillary, muscles, and LH may abolished the effects of SP on guinea pig papillary muscles.

关 键 词：休克血浆 电生理 乳头肌 益母草 

分 类 号：R285.5[医药卫生—中药学]