粗叶悬钩子根部多糖含量测定  

Extraction and Determination of Polysaccharide from Rubus alceaefolius Poir

作　　者：周建衡[1] 李天骄[1] 洪振丰[1] 胡娟[1] 

机构地区：[1]福建中医学院,福建福州350108

出　　处：《时珍国医国药》2007年第5期1055-1056,共2页Lishizhen Medicine and Materia Medica Research

基　　金：福建省自然科学基金资助项目(No.C0210020)

摘　　要：目的建立粗叶悬钩子根部多糖的含量测定方法。方法利用苯酚-硫酸法显色,标准曲线在490 nm处分光光度法测定粗叶悬钩子根部多糖含量。结果回归方程:Y=0.8325X+0.0223,r=0.9998,测得粗叶悬钩子根多糖含量9.473%,平均回收率为99.69%,RSD=0.56%(n=6)。结论该方法简便,精密度及重现性均较好,可用于粗叶悬钩子多糖的提取和含量测定。Objective To study the optimum extration process of polysaccharides from Rubus alceaefolius Poir. Methods The content of polysaccharids from Rubus alceaefolius Poir was determined by spectrophotometry at 490 nm. Results The regression equation was Y =0.832 5 × ＋0.022 3 ,r =0.999 8. The percent of polysaccharide in Rubus alceaefolius Poir was 9. 473%. The average recovery rate was 99.69% and RSD was 0.56% （ n = 6）. Conclusion The method is proved to be simple ,accurate and reliable,and it can be used to extract and determine the polysaccharides from Rubus alceaefolius Poir.

关 键 词：粗叶悬钩子 多糖 苯酚-硫酸法 分光光度法 

分 类 号：R284.1[医药卫生—中药学]