化瘀汤对大鼠子宫内膜异位症的药效学研究  

Pharmacodymanics of Dispel Stasis Decoction in rats with endometriosis

作　　者：顾静[1] 袁敏[2] 宋文成[1] 刘道芳[3] 宋礼华[3] 

机构地区：[1]安徽大学生命科学学院,安徽合肥230001 [2]安徽中医学院第一附属医院,安徽合肥230031 [3]安徽省生物研究所,安徽合肥230088

出　　处：《中成药》2007年第5期664-668,共5页Chinese Traditional Patent Medicine

摘　　要：目的:研究化瘀汤(丹参、三棱、莪术等)对子宫内膜异位症(EMS)模型大鼠TNFα-和E2水平的影响,及其抗炎镇痛作用。方法:采用手术法移植大鼠自体子宫内膜建立子宫内膜异位症模型,造模成功四周后随机分为5组:模型、高、中、低剂量组和丹那唑组,分别灌胃给药,三周后麻醉处死大鼠。进行病理组织学检查,用放免法测大鼠血清中TNFα-和E2水平。用小鼠扭体和耳廓肿胀法考察化瘀汤抗炎镇痛的作用。结果:化瘀汤组大鼠异位内膜呈不同程度的萎缩,腺体减少,血清中TNFα-和E2水平明显低于模型组。化瘀汤还对醋酸所致的小鼠扭体和二甲苯所致耳廓肿胀有较好的抑制作用。结论:化瘀汤能抑制异位内膜组织的增生,促进炎症包块的消退,并有很好抗炎镇痛作用,这可能与其抑制TNFα-分泌和E2水平有关。AIM： To observe the effects of Dispel Stasis Decoction （Radix et Rhizowa Salviae muhiorrhizae,Rhizoma Sparganii, Rhizoma Curcumae, etc） on TNF-α and E2 in rats with endometriosis（ EMS）, and to observe its anti-inflammatory and analgesic effect. METHODS： Endometriosis rat models were made by autograft of endometrium in abdomen. Four weeks after operation, EMS model rats were randomly divided into 5 groups： a model group, 3 Dispel Stasis Decoction groups and a danazol group. All the rats were treated for three weeks respectively,and then anaesthetised and killed. The ectopic endometrium＇s focuses were observed by microscope, the levels of TNF-α and E2 in the serum of all groups were measured by radioimmunoassay. The method of body twist and ear edema of mouse were used to the analysis the anti-inflammatory and analgesic effect of Dispel Stasis Decoction. RESTILTS： The survived graft shrinked and the number of the glanduls decreased in rats of Dispel Stasis Decoction group. The levels of TNF-α and E2 in the serum were significantly lower than that of the model group. Also, Dispel Stasis Decoction could inhibite the ear edema of the mouse induced by dimethylbenzene and the body twist of the mouse caused by acetic acid notablely. CONCLUSION： Dispel Stasis Decoction inhibited growth of the graft and showed a significant therapeutic effects on EMS model rat. It also has notable functions of anti-inflammatory and analgesic. This may be related to its inhibition of TNF-α and E2 levels.

关 键 词：化瘀汤 子宫内膜异位症 药效 TNF—α E2 抗炎 镇痛 

分 类 号：R285.6[医药卫生—中药学]