脐血内皮细胞对脐血早期造血细胞的体外扩增作用  

Effects of endothelial cells from cord blood on the amplification of human early hematopoietic cells from cord blood in vitro

作　　者：尹利明[1] 程腊梅[2] 王绮如[1] 谭孟群[1] 

机构地区：[1]中南大学湘雅医学院血液生理研究室 [2]中南大学干细胞研究中心,长沙410078

出　　处：《中南大学学报（医学版）》2007年第2期304-308,共5页Journal of Central South University ：Medical Science

摘　　要：目的:观察脐血内皮细胞联合细胞因子组合通过非接触方式对脐血早期造血细胞的扩增作用。方法:采用优化的内皮细胞培养基培养脐血内皮细胞;实验组分为细胞因子组合组(SCF+IL-3+IL-6+GM-CSF,CKs组)和脐血内皮细胞联合CKs非接触脐血CD34+细胞培养组(noncontact组)。Min-iMACS磁珠分选脐血CD34+细胞;检测CKs组与nocontact组培养7d后脐血早期造血细胞的扩增倍数。结果:noncontact组和CKs组都能扩增早期造血细胞,noncontact组对早期造血细胞的扩增倍数显著优于CKs组的扩增倍数。结论:脐血内皮细胞联合CKs非接触培养对脐血早期造血细胞的扩增作用显著优于CKs组。Objective To observe the effects of endothelial cells from umbilical cord blood （ UCB ） on the amplification of human early hematopoietic cells from UCB in vitro. Methods Endothelial cells from UCB were cultured by the optimized medium of endothelial cells. There were 2 experiment groups ： cytokines group （ SCF ＋ IL-3 ＋ IL-6 ＋ GM-CSF, CKs group ） and noncontact group （endothelial cell layer with CKs without contacting the CD34^＋ cells group ）. CD34^＋ cells from UCB were isolated by MiniMACS. After the cells in the CKs group and the noncontact group were cultured for 7 days, the amplifying folds of early hematopoietic cells were assayed. Results Early hematopoietic cells from UCB were expanded in the CKs group or the noncontact group. The amplifying folds of the noncontact group on early hematopoietic cells were significantly more than those of the CKs group. Conclusion The amplification effect of the noncontact group on early hematopoietic cells is superior to that of the CKs group.

关 键 词：脐血内皮细胞 CD34^+细胞 体外扩增 

分 类 号：Q813.1[生物学—生物工程]