二花脸猪和大白猪睾丸IGF-I和IGF-IR基因表达发育变化的研究  

The Developmental Changes of IGF-I and IGF-IR Gene Expression in Testicular of Erhualian and Largewhite Pigs

作　　者：李发弟[1] 夏东[1] 陈杰[1] 赵茹茜[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室

出　　处：《畜牧兽医学报》2007年第5期437-441,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金重点项目(39830290);教育部西部地区高等学校访问学者计划项目

摘　　要：为了研究生长轴激素对猪繁殖性能发育的影响,随机选取0、3、20、30、90、120、180日龄纯种二花脸公猪和大白公猪各4头,屠宰并采取睾丸组织样,以18S rRNA为内标,用相对定量RT-PCR法研究睾丸IGF-Ⅰ和IGF-IR mRNA的表达及发育性变化。结果表明,二花脸猪与大白猪睾丸IGF-ⅠmRNA表达的发育模式在30日龄前完全相同,即随着日龄的增加而呈极显著增加（P〈0.01）;二花脸猪睾丸IGF-Ⅰ mRNA相对表达量在30-180日龄无显著变化;大白猪睾丸IGF-Ⅰ mRNA相对表达量在90日龄有所下降,而在120和180日龄又恢复到30日龄水平。二花脸猪睾丸IGF-Ⅰ mRNA相对表达量显著高于大白猪（P〈0.05）。二花脸猪与大白猪睾丸IGF-IR mRNA表达的发育模式不同。二花脸猪睾丸IGF-IR mRNA相对表达量在90-120日龄呈显著下降趋势（P〈0.05）;大白猪IGF-IR mRNA相对表达量在0日龄较高,随后显著下降（P〈0.05）,并在观察期内持续保持较低水平。二花脸猪IGF-IR mRNA相对表达量显著高于大白猪（P〈0.05）。睾丸IGF-Ⅰ mRNA和IGF-IR mRNA相对表达丰度呈极显著正相关（r=0.575,P〈0.01）。结论：（1）不同品种猪睾丸IGF-Ⅰ和IGF-IR mRNA表达具有特定的发育模式;（2）猪睾丸中IGF-Ⅰ和IGF-IR mRNA的协同表达可能对猪繁殖性能的发育有重要调节作用。To understand the relationship between the IGF-Ⅰ and the porcine fertility development, testicular tissue samples were collected from male Erhualian and Large White pigs at 0, 3, 20, 30, 90, 120 and 180 days of age respectively. IGF-Ⅰ and IGF-IR mRNA level were determined by relative quantitative RT-PCR with 18S rRNA as an internal standard. The developmental pattern of testicular IGF-Ⅰ mRNA expression in Erhualian boars was similar with those in Large White boars from day 0 to 30, which increased with the age of boars. The levels of IGF-Ⅰ ,mRNA in Erhualian boars had no significant changes from day 30 to 180, while those in Large White boars showed a temporal drop at day 90 and restored to the level of day 30 thereafter （P〈0.05 ）. Overall IGF-Ⅰ mRNA level was higher in Erhualian boars than in Large White boars （P〈0.05 ）. The patterns of testicular IGF-IR mRNA expression in Erhualian and Large White boars were different. The levels of IGF-IR mRNA in Erhualian boars maintained consistent in general throughout the period of investigation, despite a tendency of decrease observed from day 90 to 120 （P〈0.05）. IGF-IR mRNA levels in Large White boars was high at day 0 and declined at day 3 （P〈0.05） , and maintained invariable thereafter. Overall IGF-IR mRNA level was higher in Erhualian boars than in Large White boars （P〈0.05）. Testicular IGF-Ⅰ mRNA level was found to be positively correlated with IGF-IR mRNA level （r=0. 575, P〈0.01）. The results suggest that IGF-Ⅰ and IGF-IR mRNA in porcine testis are coordinately expressed according to breed-specific developmental patterns, and it may play an important role in porcine testis development.

关 键 词：猪 睾丸 胰岛素样生长因子Ⅰ 胰岛素样生长因子Ⅰ型受体 发育性变化 

分 类 号：S828.2[农业科学—畜牧学]