冠心病和深静脉血栓患者蛋氨酸合成酶基因A2756G多态性检测  

Detection of methionine synthase gene A2756G polymorphism in patients with coronary heart disease and deep venous thrombosis

作　　者：齐华[1] 贺颖[1] 于海东[1] 焦昆立[2] 连建华[1] 赵洛沙[2] 郑红[1] 

机构地区：[1]郑州大学基础医学院细胞生物学与医学遗传学教研室,郑州450001 [2]郑州大学第一附属医院心内科,郑州450052

出　　处：《郑州大学学报（医学版）》2007年第3期438-440,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省高校杰出人才创新工程基金资助2005KYCX020;河南省杰出青年科学基金资助0612001300;河南省科技攻关计划项目资助0624410049;河南省骨干教师基金资助

摘　　要：目的检测冠心病(CHD)及深静脉血栓(DVT)形成患者蛋氨酸合成酶(MS)基因A2756G的多态性。方法运用PCR-限制性片段长度多态性(RFLP)对103例DVT患者、208例CHD患者和250例健康对照进行MS基因A2756G多态性分析。结果DVT组A/G+G/G型频率为10.7%,低于对照组的19.6%(χ2=4.114,P<0.05),但2组等位基因频率差异无统计学意义(P>0.05)。结论A/G+G/G型可能对静脉血栓的形成具有保护性作用,该型的分布存在种族和地域差异。MS基因A2756G多态可能不是河南汉族人群CHD发生的遗传危险因素。Aim： To investigate the relationship between the methionine synthase （MS） gene A2756G polymorphism and coronary heart disease （CHD） and deep venous thrombosis （DVT）. Methods： The MS A2756G polymorphism of 208 patients with CHD, 103 patients with DVT and 250 age- and sex-matched controls were detected using PCR,RFLP. Results： The frequencies of A/G and G/G genotypes in DVT group were 10.7% , which were lower than those in the control group（ 19.6% , χ^2 = 4.114,P 〈 0.05 ）. There were no significant differences in the frequencies of MS A2756G alleles between CHD group and the control group （P 〉 0.05 ）. Conclusion： MS A2756G A/G and G/G genotypes may have protective effect against DVT, which may vary with different ethnic groups and geographic regions. MS A2756G polymorphism may not he an independent risk factor for CHD in Henan Han population.

关 键 词：蛋氨酸合成酶 深静脉血栓 基因多态性 冠心病 

分 类 号：R593.2[医药卫生—内科学]