盐酸千金藤碱对环磷酰胺所致小鼠白细胞减少的治疗作用  

Therapeutic effects of Cepharanthine hydrochloride on leukopenic induced by cyclophosphamide in mice

作　　者：夏薇[1] 马方[1] 江金花[1] 王桂芳[1] 张小俊[1] 王庆端[1] 

机构地区：[1]河南省医学科学研究所,郑州450052

出　　处：《郑州大学学报（医学版）》2007年第3期494-496,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省属科研单位社会公益项目预研专项资金项目06411030114

摘　　要：目的观察盐酸千金藤碱(CEH)对化疗所致小鼠白细胞减少的治疗作用。方法①CEH对环磷酰胺(CTX)所致小鼠白细胞(WBS)减少的影响健康昆明种小鼠50只,随机分为5组,即正常对照组、CTX模型对照组、CEH小剂量组、中剂量组、大剂量组,每组10只。除正常对照组外,其余各组均采用剂量为75mg/(kg.d)的CTX小鼠腹腔注射,1次/d,连续3d,造成动物外周血WBC减少,于第2d开始腹腔注射生理盐水和不同剂量的CEH5mg/(kg.d)、10mg/(kg.d)、20mg/(kg.d),1次/d,连续5d。分别于用CTX前和用CTX后第3d、第5d自小鼠尾静脉采血计数外周血WBC。②CEH对CTX所致荷瘤小鼠WBC减少的影响健康昆明种小鼠50只,先接种H22实体瘤后24h,随机分为5组,即生理盐水对照组、CTX小剂量组(10mg/(kg.d))、CTX大剂量组(20mg/(kg.d))、CTX小剂量与CEH小剂量(10mg/(kg.d))合用组、CTX大剂量与CEH大剂量合用组,每组10只,进行腹腔注射,1次/d,连续8d,于第9d自各组小鼠尾静脉采血计数外周血WBC。结果①CEH对CTX所致小鼠WBC减少的影响试验中,在用CTX后第3d、第5d外周血WBC计数CEH小、中、大剂量组与单用CTX组相比差异均有统计学意义(P<0.05)。②CEH对CTX所致荷瘤小鼠WBC减少的影响试验中,CTX与CEH合用组与单用CTX组相比差异均有统计学意义(P<0.05)。结论CEH具有明显的治疗由CTX所致白细胞减少的作用。Aim ： To observe the effect of Cepharanthine hydrochloride （CEH） on leukopenic induced by chemotherapy in mice. Mothods：A total of fifty healthy KM mice were randomly divided into five groups： normal control group, cyclophosphamide （CTX） model group and C EH low-, middle-, high-dose groups, 10 mice in each group. Except the normal control group,the other groups were given 75 mg/（ kg·d） CTX intraperitoneally one time per day for 3 days to make the model of peripheral blood cell reducing in mice, then the mice in CTX model group CEH low-, middle-, high-dose grouPs were intraperitoneally treated with normal saline and CEH at different doses （5 mg/（ kg·d） , 10 mg/（ kg·d） ,20 mg/（kg·d） ） from the 2nd day, respectively, one time per day for 5 days. The peripheral leukocyte number was counted before being treated with CTX and on the 3rd day and 5th day after being treated with CTX. A total of fifty healthy KM mice were divided into five groups after they were implanted H22 cancer cells for 24 hours, they were control group （normal saline）,CTX low- and high-dose groups （ 10 mg/（ kg·d） ,20 mg/（ kg·d） ） , and the group of low-dose CTX and CEH（ 10 rag/ （ kg·d） ＋ 10 mg/（ kg·d） ） , and the group of high-dose CTX and CEH（20 mg/（ kg·d） ＋ 20 mg/（ kg·d） ） , 10 mice in each group. The mice were given drugs intraperitoneally one time per day for 8 days, then the peripheral leukocyte number was counted. Results： The total white blood cells（WBC） had siginificant difference between CTX model group and the dif- ferent-dose groups of CEH on the 3rd day and 5th day （ P 〈 0.05 ）. There were evident differences in WBC between CTX group and the groups of CTX combined with CEH in the mice bearing H22 cancer cells（ P 〈 0.05 ）. Conclusion ： The results suggest that the CEH had obvious effects in treatment of leukopenic induced by CTX in mice.

关 键 词：盐酸千金藤碱 环磷酰胺 白细胞减少 小鼠 

分 类 号：R965.1[医药卫生—药理学]