mRNA差异显示技术在分离水稻抗性基因中的应用  

Application of mRNA Differential Display Technique in Isolation of Resistant Genes in Rice

作　　者：陈昊[1] 赵森[2] 肖国樱[2] 

机构地区：[1]湖南农业大学细胞工程实验室,湖南长沙410128 [2]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《安徽农业科学》2007年第13期3820-3821,3845,共3页Journal of Anhui Agricultural Sciences

基　　金：中国科学院知识创新工程重要方向项目(KZCX3-SW-434);湖南省杰出青年基金(03JJY1004);湖南省"十一五"重大科技专项

摘　　要：mRNA差异显示技术从问世至今,在分离水稻抗性基因中得到了广泛的应用,通过对差异表达基因进行分析,能够进一步阐明水稻的抗性机理。对近年来mRNA差异显示法分离水稻抗性基因的研究做了总结,并从引物改进、PCR退火温度、dNTP浓度的改进、胶浓度的选择、标记方法的改进和差异片段验证方法的改进7个方面对mRNA差异显示技术的优化做了简单阐述。The technique of mRNA differential display has extensively been used in isolation of resistant genes in rice since its naissanee. Through the analysis of lthe differentially expressed genes, the resistant mechanism of rice could be further clarified. ＂lhe recent studies on isolating resistant genes in rice by mRNA differential display technique were summarized and the improvements of DDRT-PCR was expounded simply from 7 aspects of improvement of primer, PCR anneal temperature, improvement of dNTP concentration, selection of glue concentration, improvement of marker method and improvement of difference segment validate.

关 键 词：MRNA差异显示 水稻 抗性基因 

分 类 号：Q789[生物学—分子生物学]