珙桐芽体组织培养研究  

Research on Tissue Culture of Davidia involucrate Baill Bud

作　　者：张水成[1] 白振海[1] 张世卿[1] 

机构地区：[1]黄淮学院农林科学系,河南驻马店463000

出　　处：《安徽农业科学》2007年第13期3850-3851,3857,共3页Journal of Anhui Agricultural Sciences

基　　金：河南省教育厅自然科学研究项目(2003220095)

摘　　要：采用基本培养基、附加BA、附加IBA三因素三种浓度正交设计实验,研究了以珙桐芽作为外植体诱导其顶芽发育的过程和初代培养中愈伤组织及变异的表现。结果显示,3个因素处理下珙桐顶芽的启动率分别为14.3%、38.1%、52.4%。培养基1/2MS+BA 1.5mg/L+IBA 1 mg/L的组合效果最好,可显著提高芽的再生率。诱导出的愈伤组织种类较多,对上部芽的生长影响不同,表明新生出叶片变异程度与激素浓度相关。In tissue culture with Davidia involucrate Baill bud as explant, the design experiraents with 3 levels and 3 factors of basic medium, adding BA and IBA were used to study the development process of top buds induced by explant and callus and variation representation in first generation culture. Results indicated that the medium of 1/2 MS ＋ BA 1.5 mg/L ＋ IBA 1 mg/L got best effect in notably increasing the regeneration rate of bud. It could induce more kinds of callus and had different effect on the growth of upper buds, suggesting that the variation degree of new leaf had a relation to hormone concentration.

关 键 词：珙桐 芽体 愈伤组织 变异 

分 类 号：Q943.1[生物学—植物学]