野桑蚕羧酸酯酶基因cDNA片段的克隆及序列分析  

Cloning and Sequence Analysis of cDNA Fragment of Carboxylesterase Gene in Bombyx mandarina M.

作　　者：宋宏图[1] 李兵[1] 王东[1] 赵华强[1] 王文兵[2] 沈卫德[1] 

机构地区：[1]苏州大学蚕桑研究所苏州大学生命科学学院,江苏苏州215123 [2]江苏大学生命科学学院,江苏镇江212013

出　　处：《安徽农业科学》2007年第14期4112-4114,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"973"计划(2005CB121005);国家自然基金项目(30471309)

摘　　要：利用反转录-多聚酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)技术克隆了野桑蚕羧酸酯酶基因cDNA片段,片段长427 bp,共编码142个氨基酸残基;对来自同一发育时期的不同组织分别进行PCR扩增。结果显示:除了丝腺外其他组织均有目的条带。经与其他昆虫酯酶氨基酸同源性比较发现:野桑蚕羧酸酯酶与家蚕羧酸酯酶同源性最高,达98.6%;与赤拟谷盗同源性较低,为23.4%～30.7%。RT-PCR technology was used to clone a eDNA fragment of earboxylesterase gene in Bombyx mandarina M. The length of the eDNA fragment was 427 bp and the deduced amino acid resides was 142. PCR result in different issues at the same growth period showed that except silk gland, there were fragments in the ovarian tubule, the hemaeytopoietie organ, the fat body and the midgut. After the homologous comparison with Bombyx mori. and other insect esterases, the Bombyx mandarina M. earboxylesterase shared the highest homology （98.6 %） with Bombyx mori. earboxylesterase and lower homology （23.4 % - 30.7 %） with Tribolium castaneum.

关 键 词：野桑蚕 羧酸酯酶 克隆 RT-PCR 

分 类 号：Q78[生物学—分子生物学]