人毛囊细胞来源双层皮肤替代物的构建和组织学特征  

Reconstruction and histologic characteristics of bilayer skin substitute inoculated of human hair follicle cells

作　　者：朱堂友[1] 郝飞[1] 伍津津[2] 毕建军[2] 吴国选[2] 杨涛[2] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆400038 [2]第三军医大学大坪医院野战外科研究所皮肤科重庆市创伤愈合与组织工程研究所,重庆400042

出　　处：《第三军医大学学报》2007年第12期1187-1189,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30070701)~~

摘　　要：目的探索人毛囊细胞构建的双层皮肤替代物的组织学特征。方法用毛乳头细胞、真皮鞘细胞分别与外根鞘细胞构建复合壳多糖双层皮肤替代物,分析其体外和移植至大白鼠后的组织学特征。结果毛囊来源细胞构建的皮肤替代物中表皮细胞排列更紧密、角化突出,真皮内可见与表皮相连的上皮细胞柱及球形膨大细胞团形成。结论毛囊细胞是构建皮肤替代物的良好细胞来源。Objective To study histologic characteristics of bilayer skin substitute reconstructed by cells from human hair follicle and whether the skin appendage can be induced based on the bilayer skin substitute. Methods After composite chitosan bilayer skin substitute was reconstructed with dermal papilla cells and outer root sheath cells or dermal sheath cells and outer root sheath cells, its histologic characteristics was investigated in vitro and after transplanted onto SD albino rats. Results Composite chitosan bilayer skin substitute reconstructed by cells from hair follicle had closely arranged epithelium cells and outstanding cornification; Epithelial cords linked with epidermis could be seen in dermis. However, there was no certain hair follicle-like structure formation either in vitro or in vivo. Conclusion Hair follicle cells are good source for skin substitute reconstruction, but it can not induce skin appendage formation through skin substitute by now .

关 键 词：毛囊 毛乳头细胞 真皮鞘细胞 外根鞘细胞 皮肤组织工程 

分 类 号：R318.19[医药卫生—生物医学工程] R322.99[医药卫生—基础医学]